Paper Information

Title: 

INHIBITORY EFFECT OF GABA ON GLUCOSE STIMULATED INSULIN SECRETION FROM ISOLATED PANCREATIC ISLETS IN RAT

Type: SPEECH
Author(s): FARAJI F.*,MOTAMEDI F.,GHASEMI A.,KHOSRAVI M.,ZAHEDIASL S.
 
 *DEPARTMENT OF BIOLOGY, SCHOOL OF SCIENCE, NORTH TEHRAN BRANCH, ISLAMIC AZAD UNIVERSITY, TEHRAN
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Introduction: Gamma amino butyric acid (GABA) is an amino acid present in large quantities in inhibitory neurons of the central nervous system. It has been demonstrated in some other tissue such as islet of pancreas. The present data about the effect of GABA on insulin secretion from islets are contradictory. The aim of this study was to investigate the effect of GABA on glucose stimulated insulin secretion from isolated pancreatic islets.
Method: Pancreatic islets were isolated from 25 male Wistar rat (200-250g) with collagenase digestion technique. Insulin secretion was assessed in islets exposed to different concentration of glucose in presence or absence of GABA. Glucose concentrations were 8.3 and 16.7mM and GABA was 50mM. Islets were incubated in krebs-Ringer solution at 37oC and insulin concentrations were measured by ELISA. Each incubation cup contained eight islets and that were repeated 20 times for each condition.
Result: Insulin release was reported as mean
±SE mU/islet/time. The presence of GABA in incubation medium with a glucose concentration of 8.3 or 16.7mM showed an inhibitory effect on insulin secretion. In comparison with glucose (8.3mM) alone insulin secretion (36.6±3U/islet/60) was significantly (p<0.01) lower compared to glucose+GABA (31.7±2.4). Insulin secretion stimulated by glucose (16.7mM) in the presence of GABA (78.7±6.2) was significantly (p<0.01) lower compared to secretion in the presence of glucose 16.7mM alone (100.5±7.7).
Conclusion: This data confirm some other studies that demonstrated inhibitory effect of GABA on insulin secretion and it can therefore play a regulatory role in insulin secretion.

 
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