Paper Information

Title: 

EXERCISE-INDUCED APOPTOSIS IN SKELETAL MUSCLE AND EFFECT OF VITAMIN D3: A NOVEL TARGET

Type: SPEECH
Author(s): KUMAR V.*,GULATMONIKA I.,MUKHOPADHYAY S.
 
 *DEPT. OF PHARMACY, LOVELY INSTITUTE OF TECHNOLOGY, CHEHERU, PHAGWARA, PUNJAB, INDIA
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Background: Apoptosis is a programmed cell death which is characterized by DNA fragmentation.
Objective: The present study has been designed to investigate the effect of vitamin D in exercise-induced apoptosis in skeletal muscle of rats.
Methods: Apoptosis in skeletal muscle was induced by subjecting the rats to 8-weeks of chronic treadmill running at a speed of 36 m/min, starting from 10m/min. Vitamin D3 treatment (0.03µg/100g & 0.06µg/100g, s.c.), thrice weekly, was started three days prior to subjecting the rats to 8-weeks of chronic treadmill running and then was continued throughout the duration of exercise. At the end of 8th week the rats were sacrificed and their skeletal muscles were removed. Blood was also collected before sacrificing the rats and serum was separated. Apoptotic cell death in skeletal muscle was assessed by gel electrophoresis. Oxidative stress was measured by estimating levels of tissue TBARS, Super oxide anion, and Glutathione and serum Corticosterone level was also measured.
Results: Treadmill exercise produces DNA fragmentation in skeletal muscle, whereas Vitamin D3 treatment reduces DNA fragmentation significantly. Chronic treadmill running increases level of tissue TBARS, Super oxide anion and decreases the GSH level and Vitamin D3 treatment reduces the exercise-induced increase in level of TBARS, Super oxide anion and increases level of GHS in a dose dependent manner. Exercise also increases serum Corticosterone level whereas Vitamin D3 reduces it significantly in a dose dependent manner.
Conclusions: Thus it may be concluded that antioxidant properties of Vitamin D3 may contributes for its anti-apoptotic role in skeletal muscles.

 
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