Paper Information

Title: 

INVIVO STUDY ON TOXICITY OF A1-GM-CSF FUSION PROTEIN

Type: SPEECH
Author(s): OLOUMI M.,SHARIATI E.S.*,BOUZARI S.
 
 *MOLECULAR BIOLOGY UNIT, PASTEUR INSTITUTE OF IRAN
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Introduction: Most cancer cells are resistant to anticancer agents; one of the reasons is related to defect in induction of apoptosis because of expression of anti-apoptotic genes. In the last few years a new approach for targeted therapy of human disease has been developed using cytotoxic molecules that are produced by gene fusion techniques. Among them are Immunotoxins which comprise both the cell targeting and the cell killing moieties and is the new approach for targeted therapy of human disease.
Methods: In the present study the recombinant A1-GM-CSF produced in E. coli previously was analysed for its non-specific toxicity on C57BL/6 and BALB/c mice. In order to check this effect, the recombinant fusion protein was prepared in sufficient quantity and different amounts (10µg-500µg) was injected into mice
intraperitoneally.
Results: The data obtained indicated in all mice no death; which shows there is no non-specific toxicity due to the injection of the recombinant protein.
Conclusion: Hence it is promising result for the recombinant protein, if in future should have any application as an anti-cancer drug.

 
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