Paper Information

Title: 

EFFECTS OF GLYCLIZIDE CHITOSAN MICROSPHERES ON NORMAL AND DIABETIC RATS

Type: POSTER
Author(s): VARSHOUSAZ J.*,MINAEIAN M.,RAHDARI N.
 
 *DEPARTMENT OF PHARMACEUTICS, FACULTY OF PHARMACY, ISFAHAN UNIVERSITY OF MEDICAL SCIENCES, ISFAHAN
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Introduction: Glyclizide is a second-generation hypoglycemic sulfonylurea that is a good candidate for sustained release (SR) preparation of anti-diabetic property.
Methods: Chitosan beads of glyclizide were produced by a dispersion technique. The effects of various process were evaluated by Taguchi design on the release rate, mean dissolution time (MDT), dissolution efficiency (DE8%) and particle sizes of the microspheres. The microspheres (equal to 10mg/kg as oral suspension), glyclazide (10mg/kg as oral solution), and normal saline (5ml/kg) as well as insulin NPH (5 IU/kg, s.c.) were administered to male normal and streptozotocine (STZ) (65mg/kg) induced diabetic rats (n=8). Blood samples were taken by microhematocrite capillaries at 0, 1, 2, 3, 4, 8, 12, and 24 hours and were analyzed by standard glucose kits.
Results: The average size of microspheres was 1.45mm; loading efficiency 37.7%, MDT and DE8% were 4.83 hr and 75% respectively. Insulin NPH, glyclazide microspheres and glyclazide were effective to produce hypoglycemia in noramal rats. The efficacy was the greatest (p<0.001) for insulin compared to control group. Duration of effect was about 24 hr for insulin NPH and glyclazide microspheres however, it lasts 10 hr for oral solution of glyclazide. In diabetic rats, insulin was the only effective drug (p<0.01).
Conclusion: 1-2 % of chitosan in pH 2 and 5 and for 10 minutes of cross linking time can produce a suitable SR preparation of glyclazide which has duration of hypoglycemic action similar to insulin NPH which is significantly longer than (~14 hr) regular preparation. Lack of antidiabetic effect in glyclazide groups in STZ treated rats could be explained by the animal model used in the study.

 
Keyword(s): 
 
Yearly Visit 198   tarjomyar
 
Latest on Blog
Enter SID Blog