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Paper Information

Title: 

THE ROLES OF NITRIC OXIDE AND CALCIUM CONCENTRATION ON RELAXATION-INDUCED BY ALCOHOLIC EXTRACT PARSLEY SEED (PETROSELINUM CRISPUM) ON ILEUM'S OF ADULT MALE RAT

Type: POSTER
Author(s): MIRZAEI D.N.,MOAZEDI A.A.,SEYEDNEZHAD S.M.,ZADKARAMI M.R.,AMIRZARGAR A.A.
 
 
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Introduction: Certain medical herbs have been used instead of chemical medicines because they have fewer side effects. Petroselinum Crispum is a herb belonging to the umbelliferae family. As a medical plant parsley has been used traditionally as an antispasmodic, diuretic, emmenagogue. This work aims to provide the scientific evidence that would confirm or reject the claimed spasmolytic mechanism of parsley.
Methods: The extract was prepared from parsley seeds with 80% ethanol. At the first experiment, post mortem, 2cm of ileums adult male Wistar Rats were removed and placed in an organ bath containing Tyrode solution (37oc,PH=7.4) which bubbled with air. At the present of 100M L-NAME (Inhibitor of nitric oxide syntheses), isotonic transducer and Harvard Universal Oscillograph were used for recording contraction of the ileum after administration of 60 Mm KCl. Then cumulative concentrations of extract (0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8 mg/ml) were added to the bath. In the second experiment, 2cm of ileum were placed in an organ bath containing Tyrode solution (37oc, PH=7.4) with high concentration of K (60mM) and without CaCl2. Then at the present of each various extract (0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8mg/ml), cumulative concentrations of CaCl2 (0.225, 0.45, 0.9, 1.8, 3.6Mm) were added to the bath.
Results: Statistical analysis of first experiment showed that nitric oxide didn't influence the extract-induced relaxants in ileum (P<0.001). The results of second experiment showed that parsley extract significantly decreased CaCl2-induced contraction (P<0.001).
Conclusion: It seems that the inhibitory effect on the contractions of ileum produce by parsley seeds extract results from blocking voltage-gated calcium channels.

 
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