Paper Information

Title: 

EFFECT OF AMIODARONE ON THE CARDIAC NERVOUS GANGLIA OF RABBIT HEART

Type: POSTER
Author(s): SHAMS A.R.*,AGHAEI F.,MEHRAEIN F.,SHARIAT M.
 
 *MEDICAL FACULTY, QAZVIN UNIVERSITY OF MEDICAL SCIENCES
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Amiodarone (AD) is a drug that is used after ischemic conditions for treatment of arrhythmia and tacicardia. However this drug as a β blocker decreases arrhythmia rates but its effect on cardiac ganglia which regulate heart beat is unknown. In this research was characterized to study the morphological and ultra structural changes of neurons of cardiac ganglia after treatment with AD. 21 rabbits were selected and divided equally in two experimental and one control groups. 7 rabbits in every experimental groups of a & b were injected intra peritoneal80 mg/ kg for one or two weeks. The control group received only the same dose of normal saline serum. Then animals were anesthetized and perfused with karnovsky fixative. For electron microscopic study epicardial fat containing more in atrial and less in ventricular ganglioated nervous plexuses was removed and discarded from rabbit heart. The tissue was washed briefly in phosphate buffer saline and fixed in 2.5% glutaraldehyde and post fixed in 1% osmium tetroxide. Finally before blocking tissues stained en-block, dehydrated in graded acetone series and infiltrated in resin. Ultra structural changes in the cardiac nourons and nerve fibers of experimental rabbits in compare of control group were seen. These changes was including condensation and shrinkage of plasma lemma, appearance of electron dense bodies vacuoles in cytoplasm and formation of formation of electron mass in myelin sheath of nerve fibers. We conclude that AD treatment induces neuronal cell death and make some degenerative ultra structural changes in the cardiac neuronal cells & nerve fibers. Side effects of AM may prevent the drug from becoming a standard of therapy after ischemia.

 
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