Paper Information

Title: 

A METHOD FOR DETERMINATION OF PANTOPRAZOLE IN BIOLOGIC FLUID WITH HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

Type: POSTER
Author(s): NOUBARANI M.*,KEYHANFAR F.,MOTEVALIAN M.
 
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Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Background: The proton pump inhibitor pantoprazole is a substituted benzimidazole sulphoxide for treatment of acid-related gastrointestinal disorders.
Aim: In this study a HPLC method for determination of pantoprazole in human plasma was developed that will have application in pharmacokinetic studies.
Procedure: Following a single-step liquid-liquid extraction with tert-butyl ether/diethyl ether (70/30 v/v), pantoprazole and internal standards (zonisamide) was separated and dried under steam of nitrogen. The dried extract was reconstituted in water/methanol (50/50 v/v) and a 100µL aliquot was injected in to chromatographic system with C18 column and UV detector (290 nm). The mobile phase was a mixture of 10mM phosphate buffer (pH 6.0)/ acetonitrile (70/30 v/v) pumped at a flowrate of 1.0mL/min.
Results: With 90% extraction lower limit of quantification was 20ng/mL. A linear range of 20-5000ng/mL was established. Intra and inter variability was lower than 12%.
Discussion: This method is accurate, easy, sensitive and repeatable enough to be used in pharmacokinetic studies.

 
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