Paper Information

Title: 

STUDY OF GENE 16SRRNA VARIATION OF BISEXUAL ARTEMIA POPULATION IN IRAN BY PBR

Type: POSTER
Author(s): HAJI ROSTAMLOU M.,REZVANI GILKOLAEI S.,FATEMI SEYED MOHAMMAD REZA,SADEGHIZADEH M.
 
 
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Introduction: We used PBR method to study of genetic variation between bisexual populations of Artemia that belonging to A.urmiana and A.franciscana species.
Methods: Samples were collect5-ed from Urmia Lake in (West Azerbaijan) and Nough Pool (Kerman) and DNA was extracted by phenol-chloroform method. One pair Primer was designed based on 16SrRNA gene nucleotide sequence and used for PCR. A PCR product with 1584 pair bases in length was obtained for all specimens and subjected to digestion by 10 restriction endonuclease AluI, EcoRI, Eco47I, HaeIII, HindIII, HinfI, MboI, MspI, RsaI, TaqI.
Results: Nine of ten used enzymes were polymorphic that showed different restriction site on nucleotide sequence of gene. Totally 13 composite haplotype among 60 samples were detected (9 in Urmia and 4 in Nough). Obtained result especially high number of haplotypes indicated to considerable genetic variation among samples of Urmia Lake and Nough Pool and showed high rate of difference in nucleotide level between A.urmia and A.franciscana whose are similar to previous studies on other segment of mtDNA.
Conclusion: We conclude that different bisexual population of Artemia in Iran can be isolated using ribosomal RNA.

 
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