Paper Information

Title: 

THE EFFECT OF VITRIFICATION ON ULTRA STRUCTURAL STUDY ON LIZA AURATUS OOCYTE

Type: POSTER
Author(s): TADAYON M.,ORYAN SHAHRBANOU,SHAFIEIAN S.
 
 
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Introduction: Vitrification is the most promising method cryopreservation of fish Oocyte, Sperm and Embryos in cold area. In the present study a suitable method for protecting the mature Oocyte of Liza auratus by freezing procedure was established.
Methods: After preparing the mature Oocytes, they were divided in to three groups. freezed, vitrified and control. The vitrified groups were prepared with Ethylen-glycol. Ficoll and Sucrose. The Oocyte of two groups freezed and vitrified independently, were fixed over nylon- mesh (0.5) and exposed to the gas- Nitrogen, then 3 groups were prepared for Transmission Electron Microscopy.
Result: The results demonstrated that the protection of normal structure of control (P=0/0096) were established whilst the cellular ultra structure of freezed group were degenerated as compared with vitrificade & Control groups (P<0.001).
Discussion: since vitrification have small degeneration effects on the cell organelles, were for could preserve cell organelles from degenerating by freezing. as a result using vitrification method could be an effective and ideal way for keeping the fish Oocyte preserved and protecting it from freezing.

 
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