Paper Information

Title: 

CHARACTERIZATION AND DRUG SENSITIVITY OF A NEW PPNET/ EWING'S SARCOMA CELL LINE

Type: POSTER
Author(s): BADINLOU M.,RAJABALIAN SAEID,POURABOULI IRAN,ESKANDARI H.,JANGI AGHDAM H.,HORI M.
 
 
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Introduction: The peripheral primitive neuroectodermal/Ewing's sarcoma family of tumors (pPNET/ESFT) is highly malignant group of tumors which occur predominantly in childhood and young adults. Few established cell lines derived from pPNETs have been reported which made it difficult to examine the biological features of these tumors. In this report some characteristics of a new cell line derived from a pPNET tumor were studied. This cell line, designated as RBEJ-1, is growing in the cell culture lab of Kerman Neuroscience Research Center.
Methods: The cells were grown in Dulbecco's Modified Eagle Medium supplemented with 10% heat-inactivated fetal calf serum, 5% horse serum and 100μgr/ml streptomycin and 100u/ml penicillin at 37°C in 7% CO2. The morphological studies were done and some photographs were prepared. The cells were immunocytochemically characterized by using a panel of monoclonal antibodies. Furthermore, the chemosensitivty of the cells to cisplatin and doxorubicin anticancer drugs was assessed using MTT assay and reported by IC50 values.
Results: The morphological feature of RBEJ-1 cell line includes small round cells which grow in multilayer. The cells have been grown continuously in culture for over 40 passages. The results of immunocytochemical staining demonstrated reactivity for cytokeratin and epithelial membrane antigen proteins but no reactivity for glial fibrillary acidic protein. RBEJ-1 cells displayed the sensitivity to doxorubicin and cisplatin with IC50 values of 0.05±0.03 and 0.67±0.45 µM respectively.
Conclusion: The special biological features detailed on the RBEJ-1 cell line should make it a useful addition to the cell lines currently available for cellular and molecular studies of Ewing's sarcoma family of tumors.

 
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