Paper Information

Title:  EFFECT OF LOW-DOSE PREDNISONE IN VIVO ON THE ABILITY OF COMPLEMENT RECEPTOR TO MEDIATE AN OXIDATIVE BURST IN RAT NEUTROPHILS
Type: POSTER
Author(s): MORSHEDI A.,SAGHAEI P.,MEHRJOUYA M.*
 
 *DEPARTMENT OF IMMUNOLOGY, UREMIA UNIVERSITY
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Neutrophils are important effector cells in host defense against microorganisms. Glucocorticoids have many inhibitory effects on neutrophil functions such az chemotaxis, adhesion, transmigration, apoptosis and phagocytosis. One of the most important adverse of glucocorticoids is increased susceptibility to infections and is related to the dose, dose interval and biologic half-life of the drug administered. The mechanisms by which glucocorticoids exert these effects on neutrophils are not fully understood. The aim of this work was to study the oxidative burst mediated by complement receptors of neutrophils from rats subjected to low-dose prednisone.
Methods: 24 Wistar male adult rats weighting ± 200 g, were used in the experiments. These animals supplied neutrophils and serum to functional tests. Three groups of animals were treated with prednisone for 7 days. Within each group there were three different doses tested. The rats treated with prednisone received daily, by gavage, doses of 28, 85 and 257μg/animal during the treatment period. After the desired periods the neutrophils were isolated from whole blood.
Results: data of this study show that prednisone in all studied doses had no significant effect on oxidative burst of rat neutrophil during phagocytosis of complement- opZy particles. Observed that treatment with low doses of prednisone did not affect the NADPH-oxidase.
Discussion: Three groups of rats which received doses of 28, 85 and 257grms of prednisone for 7 days/ the maximum Comi Luminance in them of 120 / 45/ 115mv after 8 minutes was obtained. The little amount of luminance in normal control rats in each group showed very little difference near to this ligures.

 
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