Paper Information

Title: 

EFFECT OF BACLOFEN AND CGP35348 IN HIPPOCAMPUS ON TONIC PAIN SENSITIVITY DURING ESTROUS CYCLE

Type: SPEECH
Author(s): TAHERIANFARD M.,GHORBANI R.
 
 
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Estradiol and progesterone are associated with decrease on pain sensitivity. On the other hand, the role of hyppocampal GABAB receptor in nociception was investigated. So the aim of present study was to investigate the effect of interhyppocampal injection of GABAB receptor agonist (baclofen) and GABAB receptor antagonist (CGP35348) on pain sensitivity during all phases of estrous cycle. Adult female rats weighting 200-220g were maintained on 12h reversed light/dark cycle, and standard temperature 20±4. Food and water were available ad libitum. Pain sensitivity was evaluated by formalin test. Formalin test was performed by subcutaneous injection of 2.5% formalin solution into the hind paw in all phases of estrous cycle. Animal were divided in 4 groups: 1- control (intact animal), 2- sham (received 2ml Artificial cerebrospinal fluid-ACSF), 3- experiment 1 (received 2ml of baclofen 4.25 or 8.54mg/rat), 4- experiment2 (received 2ml of CGP35348 3 or 5mg/rat). Data was analyzed by two ways ANOVA measuring. The level of significant was p<0.05. Our data showed that baclofen decrease pain sensitivity in all phases of estrous cycle, but this analgesic effect was low during proestruse and estrus phase of estrous cycle and high during metestruse and diestrus. Picrotoxin increase pain sensitivity in all phases of estrous cycle, but this hyperalgesic effect was high during proestruse and estrus phase of estrous cycle and low during metestruse and diestrus. According to our data GABAergic system can modulate pain sensitivity during estrous cycle.

 
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