Paper Information

Title: 

A COMPARATIVE STUDY OF GRAPE SEED EXTRACT AND VITAMIN EEFFECTS ON SILICA-INDUCED PULMONARY FIBROSIS IN RATS

Type: PAPER
Author(s): HEMATI A.A.,NAZARI Z.,SAMEI M.*
 
 *DEPT. OF PHARMACOLOGY AND TOXICOLOGY, SCHOOL OF PHARMACY, AHVAZ JONDISHAPOUR UNIVERSITY OF MEDICAL SCIENCES
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Introduction: Due to production of Reactive Oxygen Species, oxidative stress has been implicated in the pathogenesis of silica-induced lung fibrosis. So it is hypothesized that grape seed extract (GSE) or vitamin E (vitE) as antioxidants should ameliorate some symptoms of the disease.
Methods: Male albino Wistar rats were divided into 7 groups: Rats in group I instilled intratracheally with a single dose of silica suspension (50mg/0.1ml saline/rat) as positive control (PC).Treatment groups (II-IV) received vitE (20IU/d), GSE (150mg/kg/d), or vitE and GSE simultaneously orally 1 day after instillation of silica. Groups V and VI were given GSE or vitE after instillation of the equivalent volume of saline as controls for GSE or vitE. Rats of group VII only instilled saline as negative control. After 90 days animals were sacrificed and plasma-malondialdehyde (p-MDA), lung tissue hydroxyproline (HP), serum samples BUN, creatinine, ALT, and AST levels were quantified. The lungs also were investigated for histopathological changes.
Results: The mean concentrations of p-MDA and HP in studied groups (I-VII) were 1.95, 2.77, 0.72, 0.81, 0.64, 0.94, 1.02 (µmolMDA/Lplasma) and 28.476, 27.85, 22.83, 22.64, 15.40, 18.31, and 18.51 (µgHP/gtissue), respectively. The max mean level of AST, ALT, and BUN belonged to vitE-treated and PC groups.
Conclusion: Silica caused a significant increase in HP content of lungs, p-MDA, ALT, AST and BUN levels in the samples except in GSE-treated groups (III and IV). According to the results of this study no synergistic effect was proved between GSE and vitE at the doses used.

 
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