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Paper Information

Title: 

DETERMINATION OF ATRAZINE AND AMYTRINE CONCENTRATIONS IN PRODUCED SUGAR OF AMIR KABIR FACTORY IN AHVAZ CITY

Type: POSTER
Author(s): HEMATI A.A.,NAZARI Z.,ASHTARINEZHAD A.*,TAHERKHANI K.
 
 *DEPT. OF PHARMACOLOGY AND TOXICOLOGY, PHARMACY SCHOOL, AHVAZ JONDISHAPOUR UNIVERSITY OF MEDICAL SCIENCES
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Introduction: Atrazine and amytrine are selective herbicides that are used in sugarcane fields and in many literatures, have been classified as possible human carcinogens. Thus, the determinations of them are very important for food controls.
Methods: For this purpose 30 samples of sugar of Amir Kabir Factory in Ahvaz city were taken. 20gr of each collected samples was dissolved in 50ml water. After adjusting pH to <2, by addition of phosphoric acid, to increase the herbicide retention, and the ionic strength 5gr sodium chloride was added to this solution, and mixed well then it  percolated through the conditioned C18 solid-phase extraction (SPE) cartridge column (with 10ml methanol and 10ml of Mili-Q water, respectively) under vacuum at a rate of 3ml/min. After the sample had been percolated through the column, the column washed with 5ml of Mili-Q water, the elute discarded, and the sorbent bed dried under vacuum and finally the atrazine and amytrine in sugar sample were eluted with 1ml of methanol then it was evaporated to dryness under a stream of nitrogen and the residue was dissolved in 0.5ml of mobile phase and was analyzed by HPLC using a C18 column with UV detection at 230nm, mobile phase of methanol-water (60:40) at pH 4.6 (phosphoric acid) and a flow-rate of 0.8ml/min.
Results: The mean concentrations of atrazine and amytrine in studied sugar samples were 0.1921 and 0.5559 µg/kg, respectively.
Conclusion: The results showed that all investigated sugar samples were contaminated by atrazine and amytrine.

 
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