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Paper Information

Title: 

ANTIMICROBIAL SUSCEPTIBILITIES OF BACTERIAL ISOLATES FROM CANINE OTITIS EXTERNA

Type: POSTER
Author(s): AKHTAR DANESH B.*,GHANBARPOUR R.,RAMEZANPOUR A.,HASHEMI MOGHADAM N.*
 
 *FACULTY OF VETERINARY MEDICINE, SHAHID BAHONAR UNIVERSITY OF KERMAN
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Introduction: Bacteria play an important role in Otitis externa occurrence in dogs. The aim of this study was the determination of antibiotic susceptibility of bacteria in canine otitis externa. Methods: Sterile swap samples form external ear canal were obtained from 36 unmedicated adult dogs suffering from otitis externa, processed for cytology and isolation of causative bacteria. Samples were cultured on blood agar and MacConkey agar and bacterial isolates were identified by the routine microbiology.
methods: Disc technique used for antibiotic susceptibility test by using the 12 different antibiotics, viz., penicillin G, ampicillin, gentamicin, enrofloxacin, amoxycillin/clavulanic, ciprofloxacine, oxacillin, tetracycline, trimethoprim-sulphamethoxazole, cephalexin, amikasin and polymyxin B.
Results: According to the bacteriological results Staphylococcus intermedius was most commonly species found which followed by Pseudomonas spp., beta-hemolytic streptococci, Proteus spp and Escherichia coli. All of the gram-positive bacteria tested were susceptible to amoxicillin-clavulanic acid. In contrast, no single drug was effective against all gram-negative isolates. The other gram negative isolates showed the highest sensitivity to polymyxin B, amikacin and gentamicin.
Conclusion: It was concluded that among topically used drugs, the majority of all bacterial isolates, were most susceptible to ciprofloxacin. Susceptibility of Pseudomonas spp was 100% to polymyxin B. Excluding Pseudomonas sp., the least resistance for all other isolates was seen against amoxicillin-clavulanic acid, a drug that can be used systemically. Cytology as a rapid initial method that can determine dominant organisms could be substituted by culture in uncomplicated cases.

 
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