Paper Information

Title:  THE ROLE OF GREEN-LASER IN RELEASING FREE RADICALS AND THE EFFECT OF IT ON HATCHING CHARACTERISTICS OF ARTEMIA URMIANA CYSTS
Type: POSTER
Author(s): GHADIRI A.,GHARA MALEKI H.,ALIVANDI FARKHAD S.
 
 
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Introduction: Lasers affect living organisms in different ways, for example irradiation of low intensity red laser lead to skin recovery. Also green laser cause to enzymes inactivation and stimulate the producing of free radicals. The effects of laser irradiation on living organisms depending on laboratory conditions such as: the type of treated cells, wavelength and the intensity of irradiation light are different.
Methods: In present work the effect of green laser on hatching percentage of Artemia urmiana cysts has been studied. 3 groups of decapsulated Artemia urmiana cysts irradiated by green laser Nd: Yvo4 (intensity: 1mW, wav -length: 532 nm) using a step-motor for 3, 4 and 5 hours. Treated cysts and untreated cysts (control group) incubated in conditions (27 ؛c, 2000 lux light intensity, 35 ppt sality and continuous aeration. After 24 hours incubation, hatching percentage for all groups calculated and data analyzed using SPSS V12 software.
Results: Statistic analysis showed that the hatching percentage in cysts exposed for 5 hours to the laser in comparison with control group decreased significantly (p<0.05).
Conclusion: studies show that low intensity lasers irradiations cause to produce Reactive Oxygen Species (ROS). These compounds lead to generate Superoxide Dismutase and a variety of Citokines that stimulate free radicals producing. Free radicals cause to activate cell membrane lipid peroxide and consequently increase the permeability of the cell membrane to ions like calcium. Calcium inside the cell cause to secrete liposomal enzymes and eventually cell phagocyte.

 
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