Paper Information

Title: 

THE STUDY OF PROBIOTICS EFFECTS ON 4 ENTEROPATHOGENS

Type: POSTER
Author(s): KARIMINIK A.*,ESLAMIKISH F.
 
 *DEPT. OF MICROBIOLGY, KERMAN AZAD UNIVERSITY, IRAN
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Introduction: Our intestinal tract is filled with an anormus number of helpful bacteria called probiotic bacteria. They are called probiotic bacteria because the term "probiotic" means "for life". Probiotics help us digest our food, kill harmful microorganisms and keep us functioning properly in a number of ways. Probiotics such as lactobacilli have antimicrobial effects against enteropathogens through producig organic acid, hydrogen peroxide, bacteriocins and inhibiting the cell adhesion of pathogenic organisms.
Method: In this study, 4 species of lactobacillus: L. bulgaricus (PTCC 1332), L.casei (PTCC 1608), L.plantarum (PTCC 1058) and L.fermentom (PTCC 1638) were tested for inhibitory effects on indicator pathogens: E.coli, S. aureus, Salmanella paratyphi A and Shigella dysantriae by agar overlay method. Lactobacillus species were spot inoculated onto the MRS agar and incubated at 37oC for 48h. After the formation of colonies, these plates were overlaid with 7ml of soft (0.75%) agar) TS agar containing 0.1 ml of suspension of each enteric pathogens. These plates were incubated at 37oC for 24 h. The antimicrobial activity of each lactobacillus was assessed by measuring zone of inhibition.
Results: The results showed that growth of enteropathogens were inhibited by the presence of lactobacillus.
Conclusion: Probiotics can use in chemotherapy and in the treatment of infectious disease.

 
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