Paper Information

Title: 

THE ROLE OF OPIOID SYSTEM IN SEROTONIN-INDUCED SCRATCH BEHAVIOR OF MICE

Type: POSTER
Author(s): GHAZI NEZAMI B.,GHOLIPOUR T.,EBRAHIMPOUR S.,DEHPOUR A.R.
 
 
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Background: the machanism of itching is not fully understood. While some authors believe that the neurologic basis of the itching sensation is similar to pain, and analgesic doses of morphine could suppress it; some others declare that morphine and alternatively endogenous apioids increase the behavioral response, which supports the pruritus complain in cholestatic patients, suggested to be induced by increased endogenous opioid tonus.  Serotonin induced scratching behavior in mice is used for the study of this symptom in physiological and pathological states, our objective was to investigate the involvement of opioidergic system in itching sensation by using either morphine injected Swiss mice (1mg/Kg, IP) or Bile Duct Ligated swiss mice (BDL).
Methods: Scratching behavior was videotaped by camera in a quite isolated room after intradermal injection of 0.05 ml saline containing 150nmol serotonin in the nape of the neck, and counted for 40min after the injection. The post-procedure ability for serotonin to induce scratching was considered as Morphine or BDL group members' behavior compared with its procedure-before baseline. Serotonin effect in Morphine and BDL groups was compared with serotonin effect in appropriate Sham groups, Saline injected or Sham operated mice, respectively.
Results: Data were analysed using paired t-student test and showed significant decrease of 5-HT induced scratching in both Morphine and BDL groups, compared to their baselines (mean decrease 96 and 190 respectively, both with
a<0.01), and the Serotonin effect was significantly bigger than Sham groups (mean 207 vs 15, a<0.001); this was achieved by behavior comparison of mice receiving serotonin and Saline intradermaly.
Conclusions:
Data suggest that opioid system is involved in mechanisms of itching, especially the itching of cholestasis.

 
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