Paper Information

Title: 

EVALUATING THE EFFECTS OF NEW PDE3B INHIBITORS ON INSULIN SECRETION

Type: POSTER
Author(s): HAMID F.,SHAFIEINIK R.,PARIZADEH M.R.
 
 
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Introduction: Researchers are trying to find new agents for treating diabetes mellitus with fewer side effects such as hypoglycemia. We have tested three newly synthesized inhibitors of phosphodiesterase3B, which are expected to have a stimulatory effect on insulin secretion.
Method: Male rats weighing between 250-300g were anesthetized using 80 mg/kg-IP thiopental, and pancreases were taken out. The pancreas was digested using collagenase and the islets isolated. To normalize the stressed conditions isolated islets groups, containing 5 islets, were refrigerated for 120min in a Krebs buffer containing 3mM glucose. Next, each islet group was incubated in one of the following solutions, receiving 95%O2 and 5%CO2: 3mM and 10mM glucose, 10-4M IBMX (isobutylmethylxanthine), 10-4M MCPH (methylcarbostrylphenyl), 10­­­­­-4M cilostazile and 10-4M PIP. Glucose solutions were used to observe the effects of glucose on insulin secretion, and IBMX, which is an approved general PDE inhibitor, was used to confirm the accuracy of the system. MCPH, PIP and cilostazole are the products which were tested. After the incubation, insulin concentrations were measured in supernatants.
Results: Insulin secretion in response to different solutions was as follows: 3mM glucose, 10mM glucose, IBMX, MCPH, PIP and cilostazole: 12.9, 30.9, 41.4, 33.6, 26.3 and 29.0μU/islet/h respectively (P<0.001).
conclusion: As insulin secretion in response to 10mM glucose was higher than the 3mM, and since IBMX incresed the insulin secretion comparing to 10mM glucose, we can say the system functioned well. So we can conclude that cilostazole and PIP were not able to increase insulin secretion, while MCPH could induce insulin secretion indeed and prove helpful for diabetic patients.

 
Keyword(s): 
 
Yearly Visit 16   tarjomyar
 
Latest on Blog
Enter SID Blog