Paper Information

Title:  CYTOTOXICITY EVALUATION OF FRUIT RINDS OF FOUR SPECIES OF IRANIAN CITRUS ON HELA CELL LINE
Type: POSTER
Author(s): KHODA RAHMI GH.A.*,GHANADI A.R.,HASANZADEH F.,BIDOKHTI M.
 
 *DEPT. OF PHARMACEUTICAL CHEMISTRY, ISFAHAN UNIVERCITY OF MEDICAL SCIENCES
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Introduction: Cytotoxic effects have been demonstrated for flavonoids on several human cancer cell lines. As citrus fruits are important sources of flavonoids, cytotoxic effects of fruit rinds of four species of Iranian citrus including C. reticulata hybrid (Bakraee), C. medica (Balang), C. grandis (Darabi) and C. japonica (Porteghal Japoni) were evaluated.
Methods: Fruits of four species of Iranian citrus were collected from khafr, Fars and Sari, Mazandaran. Total extracts were prepared by maceration with ethanol 70° while enriched flavonoid fractions were successively extracted by soxhlet method with ethyl acetate. Then, the cytotoxic effects of these extracts against Hela cell line were evaluated. Briefly, Hela cells were seeded at 2-5104 cells / well in 96-well plates and after 24hr incubation, treated with 8 concentrations (20, 50, 75, 100, 125, 150, 175, 200 µg/ml) of the extracts. Then, viability of cells was determined by reduction of MTT to formazan following further 48hr incubation. The absorbance was measured at 540nm using an ELISA plate reader.
Results: Total extracts of Bakraee, Balang, Darabi and Porteghal Japoni didn't show significant cytotoxic effects at 20-200 µg/ml concentrations, while the enriched flavonoid fractions of these plants showed moderate cytotoxic effects.
Discussion: The IC50s of the enriched flavonoid fractions of Bakraee, Balang, Darabi and Porteghal japoni were 192, 188, 154 and 145µg/ml, respectively. Porteghal japoni showed the most cytotoxic effects in this study.

 
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