Paper Information

Title: 

CYTOTOXIC EFFECTS OF ESSENTIAL OILS OF SOME IRANIAN CITRUS PEELS

Type: POSTER
Author(s): JAFARIAN ABAS,GHANDI A.R.,MONAJEMI R.,ORYAN SHAHRBANOU,HAERI ROUHANI SEYED ALI
 
 
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

In this study we sought to determine the cytotoxicity of Citrus limon and C. sinensis essential oils. Essential oils of Iranian Citrus limon (L.), C. sinensis (L.) peels, were prepared by hydrodistilation and characterized by GC-MS. The effects of C. limon (5-40 µg/ml) and C. sinensis (0.25-10 µg/ml) on two human tumor cell lines (MCF-7 and Hela) were determined. Different concentrations of essential oils were added to cultured cells and cell survival was evaluated using MTT-based cytotoxicity assay. Briefly, cells were seeded and different amounts of essential oils were added. Cells were incubated for 72 h and their survival was evaluated by addition of tetrazolium salt solution. After 3 h medium was aspirated, DMSO was added and absorbance was determined at 540 nm with an ELISA plate reader. Cytotoxic effect was considered when more than 50% reduction in cell survival was observed. Doxorubicin, a known cytotoxic drug, was used as a positive control. Limonene comprised about 98.4% and 98.8% of content of C. limon and C. sinensis essential oils, respectively. IC50 of essential oil for MCF-7 cell line was: C. limon ≈ 10 µg/ml and C. sinensis ≈ 0.5 µg/ml. For Hela cell line IC50 was: C. limon ≈ 17 µg/ml and C. sinensis ≈ 3 µg/ml. Our findings revealed that C. limon and C. sinensis had more cytotoxic effect on MCF-7 than that on Hela cells. Also comparing IC50, our findings indicated that C. sinensis was more cytotoxic than C. limon.

 
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