Paper Information

Title: 

THE CYTOTOXIC EFFECTS OF PERSICA MOUTHWASH ON CULTURED HUMAN AND MOUSE CELL LINES

Type: POSTER
Author(s): MOHAMMADI M.,RAJABALIAN SAEID,MOZAFARI B.
 
 
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Background and Aim: The effectiveness of an ideal antimicrobial agent depends on its ability to kill microbes with minimal toxicity to host cells. Several studies have been reported on the antimicrobial effects of chewing sticks (Salvadora persica L.) on oral bacteria. The purpose of this study was the evaluation of cytotoxic effects of Persica mouthwash on cultured human and mouse cell lines.
Methods: This was an experimental study. In this study, toxic effects of persica and chlorhexidine mouthwashes on the KB, Saos-2, J744 A1 and gingival fibroblast cells were evaluated by MTT assay. Moreover, the effect of fetal calf serum (FCS) components on cytotoxicity activity of these mouthwashes was investigated.
Statistical analysis:  Analyses of variance and Kruskal-Wallis were used to evaluate the results of this study.
Results: These results indicated that concentrations of higher than 0.1% of Persica exerted a very significant cytotoxic effect on all cell lines (p<0.01 to p<0.001). CHX at concentration of 0.001% exerted toxic effects only on gingival fibroblasts. However, CHX concentrations of higher than 0.001% were required to produce significant cell death in the other cell lines. Furthermore, in all the concentrations under study, both Persica and CHX exerted significant cytotoxic effects in the absence of FCS in comparison to control culture medium. The drug's toxicity was attenuated in the presence of FCS (10%).
Conclusion: Our results indicated both persica and CHX mouthwashes are toxic to Macrophage, Epithelial, Fibroblast and Osteoblast cells in a concentration dependent manner.

 
Keyword(s): 
 
Yearly Visit 8   tarjomyar
 
Latest on Blog
Enter SID Blog