Paper Information

Title: 

EXPERIMENTAL EFFECT OF DIAZINON ON PITUTITARY GLAND AXIS AND TESTIES HISTOLOGICAL CHANG ON ADULT RAT

Type: POSTER
Author(s): RAHIMI S.,RAHIMI A.,AMIRI J.
 
 
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Diazinon, an organophosphate insecticide, is widely used in agriculture worldwide and also in Iran. Extensive application of diazinon leads to environmental pollution and its entrance into the food-chain could have detrimental effects on human and other species. Diazinon inhibits the enzyme acetylcholinesterase. In this experiment the effects of diazinon on reproductive parameters of the male Wistar rats were investigated. Mature male Wistar rats (n=80) were randomly allotted into 5 groups (n=8 rats per group). The rats of three groups received either 50 (D50 group), 100 (D100) or 150 g (D15) diazinon per kg live weight per day, dissolved in normal saline. The other groups were left untreated (Control 1) or received an equivalent amount of normal saline without diazinon (Control 2). The rats were euthanized after 28 days, and a blood sample was collected via heart puncture for determination of serum testosterone concentration. The testes were then removed for histological studies. The data were statistically analyzed by using the GLM procedure of the SAS system, and the mean comparisons were performed by using the Duncan's multiple range test. Diazinon treatment did not have a significant effect on the body and testis weights but significantly (P<0.05) decreased serum testosterone concentration and the numbers of Sertoli cells, Leydig cells, primary spermatocytes and spermatids. Decreased testosterone concentration in blood may be due to inhibition of synthesis of the steroidogenic acute regulatory protein (StAR) in the testis. The results indicated that diazinon, at the levels employed in this experiment, can adversely affect the reproduction in the male.

 
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