Paper Information

Title:  ACTIVATION OF NICOTINIC ACETYLCHOLINE RECEPTORS MODULATE 4-AMINOPYRIDINE INDUCED EPILEPTIFORM ACTIVITY IN RAT HIPPOCAMPAL SLICES
Type: POSTER
Author(s): ROUSHAN MILANI S.*,KHOSHNOUD MANSOUR KHANI M.J.,COBB S.R.
 
 *DEPT.OF PHYSIOLOGY, UROMIEH MEDICAL SCIENCE UNIVERSITY
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Introduction: Nicotinic acetylcholine receptors (nAChRs) regulate neuronal excitability within the CNS and may be relevant to several forms of idiopathic epilepsy. It has recently been found that certain forms of epilepsy may arise from mutation in the genes responsible for encoding of nicotinic acetylcholine receptor subunits. Moreover, many reports have shown that high doses of nicotine induce seizures in animals, which are blocked by different nAChR antagonists. However, the mechanism underling the role of nAChRs in patterning epileptiform activity is poorly understood. In the present study, we investigated the effects of nAChR ligands on 4-aminopyridine (4-AP) induced epileptiform activity in rat hippocampal slices to assess the effectiveness of nAChRs in modulating epileptiform activity.
Methods: Horizontal Wistar rat (2-5 weeks old) hippocampal slices (400
mm thick) were prepared using standard procedures and placed at an artificial CSF/ humidified oxygen interface. Extracellular recordings were obtained in the area CA3 using glass microelectrodes (1-5 MW) filled with 2 M KCl. Data were captured as a continuous data stream using AxoScope 8. All pharmacological agents were applied to the bath from frozen stock solutions. Data was analysed off-line using p-Clamp, Origin and Prism software. All values expressed as mean±standard error.
Results:
nAChR agonists application induced burst frequency potentiation which were reversed upon washout of the agonists or co-application of nAChR antagonists. These results provide compelling evidence that activation of nAChRs exacerbate epileptiform activity in the rat hippocampus.
Conclusion:
nAChRs modulate epileptiform activity in the hippocampus and more than one subtype of nAChR may be involved.

 
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