Paper Information

Title: 

PARAQUAT - INDUCED HEPATOTOXICITY IN THE RATHEPATOCYTES

Type: SPEECH
Author(s): ELMI S.*,SADEGHI ZH.,ELMI A.,DARAIE B.,GHAZI KHANSARI M.
 
 *DEPARTMENT OF PHARMACOLOGY, SCHOOL OF MEDICINE, TEHRAN UNIVERSITY OF MEDICAL SCIENCES, TEHRAN, IRAN
 
Name of Seminar: IRANIAN CONGRESS OF TOXICOLOGY
Type of Seminar:  CONGRESS
Sponsor:  SOCIETY OF TOXICOLOGY
Date:  2004Volume 8
 
 
Abstract: 

The wide use of herbicide paraquat in recent year demands an effort to evaluate the toxicity mechanisms exerted on mammalian cell metabolism. The aim of this study was to evaluate the hepatotoxic effects of different doses of herbicide paraquat (1, 1’-dimethyl-4, 4’-bipyridylium dichloride) using rat hepatocytes.
Hepatocytes were isolated from male Wistar rats (200-250grams) following two steps collagenase method. Isolated cell populations which contained more than 85% viable cells were used in 4 groups: 1) saline control, 2) paraquat (1mM) 3), paraquat (5mM), 4) paraquat (10mM). These group containing 1x10-6 cells were incubated in rotating-round bottomed flask at 37ºC under carbogen (5% CO2 and 95%O2) for 3 hours. Samples were withdrawn at different time intervals for the analysis of the viability. Lactate dehydrogenase (LDH) leakage and GSH levels were determined at the end of incubation period. All doses increased LDH leakage level but there was a significant difference in LDH leakage at the doses of 5&10mM (p<0.05) in compare to control group. Incubation of isolated hepatocyte in the presence of herbicide resulted in loss of intracellular glutathione. Paraquat exerted its maximal effect on GSH level at 10mM concentration. It is concluded that paraquat toxicity in hepatocyte cells is time and concentration dependence. Since GSH is known to play an important role in protecting cells from oxidant induced tissue injury the result indicated that the herbicide paraquat may induce cell death by decreasing cellular GSH level
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