Paper Information

Title: 

COMPARISON OF RAST AND UNICAP 100 IN DETERMINING OF COW'S MILK PROTEIN SPECIFIC IGE

Type: PAPER
Author(s): SHOKOUHI SHOUR MASTI R.*,POURPAK Z.,AKRAMIAN R.,MANSOURI MAHBOUBEH,KAMALI SAMINEH
 
 *IMMUNOLOGY, ASTHMA AND ALLERGY RESEARCH INSTITUTE, CHILDREN MEDICAL CENTER, MEDICAL SCIENCES/UNIVERSITY OF TEHRAN, TEHRAN, I.R., IRAN
 
Name of Seminar: BIENNIAL CONGRESS ON IMMUNOLOGY, ASTHMA AND ALLERGY
Type of Seminar:  CONGRESS
Sponsor:  IRAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES
Date:  2007Volume 6
 
 
Abstract: 

Background: UniCAP is an automatic autoanalyser that can be used for determining total and specific IgE in allergic diseases, and RAST (Radioallergosorbent test) is a classical technique for specific IgE.
Objective: The objective of this study was to compare the results obtained from RAST and CAP system in serum patients with Cow's milk allergy.
Materials and Methods: The population of this study consisted of 24 patients with cow's milk allergy. All the patients had clear history of anaphylaxis to cow's milk protein. A blood sample was taken from each patient and both RAST (Allergopharma) and UniCAP 100 (Phadia) were performed for determining specific IgE to cow's milk protein (on the basis of KU/L and Grade 0-5). Finally, the results were compared with t test.
Results: Our patients were 15 males and 9 females. The values for Cow's milk specific IgE determined by RAST ranged from 0 to 71.00 KU/L (mean =5.85KU/L, Grade= 0-4) and by UniCAP 100 were from 0.25 to 95.40 (mean=18.69KU/L, Grade=0-5). A significant difference (P=0.005) was between mean of specific IgE in methods of RAST and UniCAP 100.
Conclusion: It seems that UniCAP is more precise than RAST in determining specific IgE in serum of patients with cow's milk allergy. It can owe solid phase of UniCAP system.

 
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