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Paper Information

Title: 

COMPARATIVE STUDY OF ALLERGENIC POLLEN’S PROTEIN CONTENT IN TWO MAJOR VARIETIES OF CUPRESSUS ARIZONICA PLANTED IN TEHRAN

Type: PAPER
Author(s): MAJD AHMAD,SHAHALI Y.*,POURPAK Z.,MOEIN MOSTAFA,TAJADOD G.
 
 *DEPARTMENT OF BIOLOGY, NORTH BRANCH OF TEHRAN, ISLAMIC AZAD UNIVERSITY, TEHRAN, IRAN
 
Name of Seminar: BIENNIAL CONGRESS ON IMMUNOLOGY, ASTHMA AND ALLERGY
Type of Seminar:  CONGRESS
Sponsor:  IRAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES
Date:  2007Volume 6
 
 
Abstract: 

Introduction: Cupressus arizonica has been abundantly planted in numerous countries causing a signifiant augmentation of allergy diseases from the middle of winter to the beginning of spring.
Objective: The aim of this study was to compare pollen protein content in two major varieties of C.arizonica planted in Tehran, C.arizonica var.arizonica and C.arizonica var.glabra, in order to determine pollen’s specificity of each variety.
Materials and Methods: The Pollen grains were directly collected from mature male cones of trees planted in different areas of the city. Pollen’s proteins were extracted in 0.01M PBS (pH 7.4), and were analysed by SDS PAGE. Total protein content of extracts was measured and compared via Bradford assay.
Results: Our investigations revealed an appreciable difference in pollen protein contents of each variety and a new major protein, with an approximate molecular weight at about 33 kD has been detected for the first time in both varieties.
Discussion:There is some intraspecie specificities in Arizona cypress pollen extracts. The major allergen of Cupresuss Arizonica pollen, Cup a 1 (45 kD), has been reported as the most representative protein in pollen extracts of mediterranean countries, but in our autochtone extracts of both varieties, a protein band at 33 kD was more representative. These observations seem to indicate some relative differences of C.arizonica pollen protein content in the different environmental and climatic conditions of Tehran and mediterranean countries. The confirmation of this aspect would facilitate the preparation of an effective extract improving the diagnosis of the allergy to Cupressus arizonica.

 
Keyword(s): ARIZONA CYPRESS, CUPRESSUS ARIZONICA POLLEN, INTRASPECIE SPECIFICITIES, POLLEN EXTRATC, SEASONAL ALLERGIES, POLLEN PROTEIN CONTENT, SDS-PAGE, ECOTYPE
 
 
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