Paper Information

Title: 

RELAXANT EFFECT OF CAROTA AQUEOUS EXTRACT ON RAT ILEUM

Type: POSTER
Author(s): ABBASIASL H.*,AMIR ZARGAR A.,AHANGARPOUR A.,ZAMANPOUR Z.,HASANZADEH MINA
 
 *DEPARTMENT OF PHYSIOLOGY, PHYSIOLOGY RESEARCH CENTER & DIABET RESEARCH CENTER, SCHOOL OF MEDICINE, JUNDISHAPOUR MEDICAL SCIENCES UNIVERSITY OF AHWAZ, AHWAZ, IRAN
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2009Volume 19
 
 
Abstract: 

Daucus carota L. is from Apiaceae alt. Umbelliferae and its seed and root are commonly used. It is useful in the treatment of cough, dysentery, cancer, malaria, tumors and kidney stones and diarrhea. The aim of present study was to investigate the antispasmodic effect of carrota aqueous extract on rat ileum contractility. Virgin rats from Wistar descent 200-300g were kept. After laparatomy, a piece of ileum (1.5cm) was excised and mounted in an organ bath (10ml) containing Tyrode solutions (37oC) under 1g tension. KCl (60mM) and BaCl2(4mM) induced ileum contraction by transducer isotonic and after 2 min and during the plateau, the extract was added at (0.05, 0.1, 0.2 and 0.4 mg/ml) and cumulatively to the organ bath with 10 min intervals and no refreshing bath solution and the contractions was perused. The new portions of the ileum were separately incubated with propranolol (1μM, 30min), naloxone (1μM 30min) and the extract was added at (0.05, 0.1, 0.2 and 0.4 mg/ml) cumulatively to the organ bath. Furthermore, in Ca2+ -free, rich KCl (60mM) Tyrode solution, the tissue contracted by cumulative concentration of CaCl2 (0.225-0.27mM) before tissue incubation (3min) with extract (0.5, 0.1, 0.2 and 0.4 mg/ml) non cumulatively. Results showed that cumulative concentrations of the extract reduced the basal and peak of ileum contractions (induced by KCl and BaCl2), dose dependently (P<0.001). Incubation of the tissue with naloxone and propranolol were not changed the spasmodic effect of the extract. Extract reduced the ileum contraction induced by CaCl2, dose-dependently (p<0.01). It can be concluded that Daucus carota L extract inhibits contractions without involving β-adrenoceptor and opioid receptor but with involving potassium channels. It is suggested that this antispasmodic effect may also be via calcium channels receptors.

 
Keyword(s): CARROTA, PROPRANOLOL, NALOXONE
 
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