Paper Information

Title: 

INOSINE ATTENUATES TNBS-INDUCED CHRONIC COLITIS IN RATS; POSSIBLE ROLE OF URIC ACID

Type: POSTER
Author(s): RAHIMIAN R.*,FAKHFOURI G.,DANESHMAND A.,MOUSAVIZADEH K.,DEHPOUR A.R.
 
 *DEPARTMENT OF PHARMACOLOGY, SCHOOL OF MEDICINE, TEHRAN UNIVERSITY OF MEDICAL SCIENCES, TEHRAN, IRAN
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2009Volume 19
 
 
Abstract: 

Inflammatory bowel disease (IBD) is a multifactorial disease with an unknown ethiology, characterized by the oxidative stress, leucocyte infiltration and rise in inflammatory cytokines such as TNFa and IL1b. Intestinal lesions are associated with neutrophil infiltration and high levels of malondialdehyde, a marker of lipid peroxidation. Protective effects of inosine, uric acid precursor, possessing direct anti-inflammatory properties have been successfully investigated in experimentally and clinically inflammatory conditions such as multiple MS, bacterial meningitis and spinal cord injury. In this study we have investigated the effects of Inosine on Trinitrobenzene sulfonic acid (TNBS)-induced colitis in rats. Experimental colitis was induced in male rats by delivering TNBS to the distal colon. Inosine(500mg/kg, ip), Oxonic acid (250mg/kg, ip), a uricase inhibitor, and Inosine plus Oxonic acid was administered once a day from 2h prior to induction of colitis and continued for 6 successive days thereafter. Colonic status was investigated throughmacroscopic, histological and biochemical analyses 6days following colitisinduction. Results: amelioration of the morphological signs including macroscopic damage, necrotic area, and histology were seen subsequent to treating animals with inosine, oxonic acid and inosine plus oxonic acid. These observations were accompanied by a significant reduction in the degree of both neutrophil infiltrations, indicated by decreased myeloperoxidase activity, and lipid peroxidation, asmeasured by a decline in malodialdehyde content in inflamed colon as well as adecrease in levels of inflammatory cytokines (TNFa, IL1b). In conclusion, these findings suggest that inosine exerts beneficial effects on the TNBS-induced colitis model.

 
Keyword(s): INOSINE, INFLAMMATORY BOWEL DISEASE, ULCERATIVE COLITIS, MYELOPEROXIDASE, MALONDIALDEHYDE, INFLAMMATORY CYTOKINES
 
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