Paper Information

Title: 

EFFECTS OF SMOKING AND HYPERCHOLESTEROLEMIA ON DIFFERENT PARAMETERS OF OXIDATIVE STRESS IN BLOOD

Type: POSTER
Author(s): ARDI P.*,FIROUZI OMID REZA,SAADATI H.R.,MIRI RAMIN
 
 *MEDICINAL AND NATURAL PRODUCTS CHEMISTRY RESEARCH CENTER, SHIRAZ UNIVERSITY OF MEDICAL SCIENCES, SHIRAZ, IRAN
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2009Volume 19
 
 
Abstract: 

Oxidative stress (OS) is caused by an imbalance between the production of reactive oxygen species (ROS) and the ability of biological systems to detoxify these species. ROS, including free radicals, are useful in normal conditions and are involved in normal biochemical processes, but their overproduction can cause damage to various macromolecules. OS is involved in the pathology of many diseases such as atherosclerosis andcancer. Smoking is a common mechanism of OS and is believed to cause inpart its harmful effects through OS. The aim of this study was to evaluate different aspects of OS on various macromolecules. We also sought to evaluate the effect of hypercholesterolemia on parameters of OS and the comorbidity of smoking and hypercholesterolemia. Blood samples were collected from volunteers. Subjects were divided to 4 groups; nonsmokernormocholesterolemic, nonsmoker-hypercholesterolemic, smokernormocholesterolemic, smoker-hypercholesterolemic. Several parameters of OS were assessed in the blood. Levels of antioxidant enzymes, superoxide dismutase (SOD), glutathione peroxidase (GPX) and catalase (CAT) were measured in erythrocytes by appropriate spectrophotometric methods. Total antioxidant activity of plasma was measured by the Ferric Reducing Antioxidant Power (FRAP) in plasma. Protein oxidation was assessed by evaluation of the levels of sulfhydryl groups by Ellman’s method. Lipid peroxidation was quantified by determination of total and free isoprostane F2a (IPF2 a) levels in plasma with a commercial EIA Kit. Results showed that some of the parameters of OS such as IPF2 a, FRAP and GPX were changed in smokers. The effect of hypercholesterolemia alone or as a comorbidity factor with smoking was also evaluated with statistical tools. In conclusion, smoking affects various macromolecules in different ways and the simultaneous presence of hypercholesterolemia can modulate the effectsof smoking on OS parameters.

 
Keyword(s): SMOKING, HYPERCHOLESTEROLEMIA, OXIDATIVE STRESS
 
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