Paper Information

Title: 

PROTECTIVE EFFECT OF β-CAROTENE ON PROTEIN AND ELECTROLYTE URINARY EXCRETION AFTER THE ISCHEMIA/REPERFUSION INDUCED DAMAGE IN THE RAT KIDNEY

Type: POSTER
Author(s): GHAFARI M.A.*,HOSSEINI F.,GHARIB NASERI MOHAMMAD KAZEM,BADVI M.,SHAHBAZIAN H.,RASHIDI I.
 
 *DEPARTMENT OF BIOCHEMISTRY, SCHOOL OF MEDICINE, AHWAZ JUNDISHAPUR UNIVERSITY OF MEDICAL SCIENCES, AHWAZ, IRAN
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2009Volume 19
 
 
Abstract: 

Renal ischemia is a major cause of acute renal failure which remains a major clinical problem with high prevalence and a mortality rate of up to 60% in critically ill patient. Reactive oxygen species are suggested to participate in ischemia/reperfusion (I/R) injury in the kidney. The urinary excretion of protein and electrolyte is the most important biochemical symptom of this disorder. Thus this study was designed to investigate the effect of β-carotene on protein and electrolyte urinary excretion in I/R induced renal injury in the rat kidney.
Male adult Wistar rats were exposed to 45 min of renal ischemia followed by 4 h of reperfusion. Either β-carotene (10, 30 and 100 mg/kg) or saline was administrated (i.p.) 5 days prior to ischemia. At the end of the reperfusion period, renal function was assessed by urinary analysis. I/R caused a significant increase in the levels of urinary excretion of protein, Na+, K+ and Ca+2 in rats compared with the control group. Pretreatment of rats with β-carotene (100 mg/kg/i.p.) significantly reduced protein and Ca+2 urinary levels, and at doses 30 and 100 mg/kg/i.p. also reduced Na+ and K+ levels (dose dependent manner) in urine compared with the control group.
Findings of this study revealed that β-carotene pretreatment at the dose of 100 mg/ kg/i.p was more effective than 10 or 30 mg/ kg/i.p. in ameliorating the urine biochemical parameters after renal I/R injury. Therefore we suggest that reactive oxygen species(ROS) may play a causal role in I/R induced renal injury, and that β-carotene exerts renal-protective effects probably by inhibiting ROS and antioxidant activities.

 
Keyword(s): β-CAROTENE, PROTEIN, ELECTROLYTE, ISCHEMIA/REPERFUSION, RAT, KIDNEY
 
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