Paper Information

Title:  THE EFFECTS OF PRETREATMENT WITH LOW DOSE LPS ON KIDNEY AND LIVER DAMAGE IN RENAL ISCHEMIA-REPERFUSION INJURY
Type: POSTER
Author(s): NAJAFI ATEFEH*,KADKHODAEI MEHRI,DELAVARI F.,SEYFI B.,SHAMS SEDIGHEH,KHASTAR H.
 
 *DEPARTMENT OF PHYSIOLOGY, SCHOOL OF MEDICINE, TEHRAN UNIVERSITY OF MEDICAL SCIENCES, TEHRAN, IRAN
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2009Volume 19
 
 
Abstract: 

Ischemia-reperfusion (IR) induces inflammatory responses in different organs and produces reactive oxygen species (ROS) that induces local and distantorgan injuries. There are many reports on the liver changes because of renal IR injury. Recent studies demonstrate that LPS-preconditioning increases resistance of myocardial tissues against subsequent IR injuries. The aim of the present study is to assess the protective effect of low dose LPS on kidney and liver damage after induction of renal IR injury.
Male Sprague-Dawley rats were randomly assigned into two groups, LPS pretreatment group in which LPS was injected (0.2mg/kg, i.p) 24 hours before induction of renal IR and control non-pretreated group (normal saline injection). Bilateral renal ischemia was conducted by 45 minute clamping of both renal arteries followed by 1-hour reperfusion. At the end of the experiments, blood samples were collected for renal and liver functional measurements.
There were no significant differences in renal functional indices including BUN & serum creatinine and liver functional indices including ALT (Alanine Transferase), AST (Aspartate Transferase) and ALK (Alkaline Phosphatase) between two groups in this model of renal IR injury.
Pretreatment with low dose LPS has no protective effect in prevention of renal bilateral IR injuries (45 minute ischemia, 60 minute reperfusion). Since 1-hour reperfusion may not be long enough for achievement of protective effects of the treatment, further studies are needed for evaluation of renal and liver function in longer reperfusion periods.

 
Keyword(s): ISCHEMIA-REPERFUSION, LIPOPOLYSACCHARIDE PRECONDITIONING, KIDNEY, LIVER
 
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