Paper Information

Title: 

THE ROLE OF A1-ADENOSINE RECEPTOR IN PROGRESSION OF RENAL DYSFUNCTIONS DURING 24 HOUR OF REPERFUSION FOLLOWING ISCHEMIA IN ANAESTHETIZED RATS

Type: POSTER
Author(s): NAJAFI H.*,SHID MOUSAVI SEYED MOSTAFA
 
 *DEPARTMENT OF PHYSIOLOGY, SCHOOL OF MEDICINE, SHIRAZ UNIVERSITY OF MEDICAL SCIENCES, SHIRAZ, IRAN
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2009Volume 19
 
 
Abstract: 

The objective of present study is to find out the role of A1-adenosine receptor (A1-AR) in the progression of disturbances in renal hemodynamics, excretory function, urine concentrating ability and acidbase regulation during the course of 24-h reperfusion following ischemia. Male Sprague-Dawley rats were lightly anaesthetized and subjected to bilateral renal artery clamping for 30 min. The reperfusion periods of 4, 12 and 24 h were chosen in the I/R-4h, I/R-12h and I/R-24h groups, respectively. A selective A1-AR antagonist (DPCPX, 2 mg/kg) was injected at 30 min before renal ischemia in the I/R-4h+D, I/R-12h+D and I/R 24h+D groups. There were also sham-4h, sham-12h and sham-24h groups, in which renal arteries were not occluded (n=6-7 in each group). Two hours before the end of reperfusion period in each group, rats were reanaesthetizes for cannulations and a clearance period was performed in the last 1-h of reperfusion, during which urine was collected under 30-mm paraffin and arterial blood samples were taken at the beginning and end of it. Comparisons of the results in I/R-4h, I/R-12h and I/R-24h groups with their own sham groups showed large decreases in creatinin clearance, absolute potassium excretion, free-water reabsorption, and plasma bicarbonate concentration, as well as, increases in fractional sodium and bicarbonate excretion and plasma creatinin concentration (P<0.05-0.001). The levels of all these variables were well improved in the I/R-4h+D and I/R-12h+D groups, but most of them deteriorated in the I/R-24h+D group. Therefore, the role of endogenous activation of A1-AR in the development of renal ischemia/reperfusion-induced dysfunctions is contributive during early hours but protective at the later 24-h time frame of reperfusion.

 
Keyword(s): ACUTE RENAL FAILURE, ISCHEMIA/REPERFUSION, A1-ADENOSINE RECEPTOR, RENAL FUNCTION
 
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