Paper Information

Title: 

INTERACTION OF ADENOSINE A1-RECEPTOR AND INDUCIBLE NITRIC OXIDE SYNTHASE ON TISSUE DAMAGE AND FUNCTIONAL DISORDERS AT EARLY PHASE OF RENAL POST-ISCHEMIA IN ANAESTHETIZED RATS

Type: POSTER
Author(s): GHOLAMPOUR F.*,SHID MOUSAVI SEYED MOSTAFA,OUJI S.M.*,HAJIZADEH S.
 
 *DEPARTMENT OF PATHOLOGY, SCHOOL OF MEDICINE, SHIRAZ UNIVERSITY OF MEDICAL SCIENCES, SHIRAZ, IRAN
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2009Volume 19
 
 
Abstract: 

The aim of this study was elucidating the role of adenosine A1-receptor and iNOS as well as their interacton on development of tissiue damage and functional disorders of kidney at early phase of reperfusion following bilateral renal ischemia. After surgery and cannulations in pentobarbital anaesthetizedmale sprague-dawley rats (270-320 g), a 30-min control clearance period was taken. Following 30-min of renal arteries clamping, 4 consecutive 30-min clearance periods were performed, and finally both kidneys were removed and preserved. From 45 min before ischemia period, rats were given the i.v. infusions of DPCPX (200 mcg/kg bolus, and then 10 mcg /kg.min) as a selective A1-receptor antagonist in the I/R+D group, l-NIL (3 mcg /kg bolus, and then 1 mcg /kg.h) as a selective iNOS inhibitor in the I/R+N group, DPCPX and l-NIL together in the I/R+D/N group, normal saline in the I/R group, and normal saline without occlusion of renal arteries in the sham group. The total renal histopathological scores in the groups of I/R (61.0±0.4), I/R+D (30.0±1.7), I/R+N (26.0±2.0), I/R+D/N (17.0±0.8), and sham (0) were different from each other’s (all p<0.05). in the I/R group, there were progressive reductions in creatinine clearance, sodium reabsorption, potassium excretion and free-water reabsorption during the last three 30-min of reperfusion, which administrations of L-NIL more than DPCPX and both together more intensively improved these disorders. Therefore, iNOS a had more considerable role than adenosine A1- receptor, the two having a significant interaction in the induction of tissue damage and disorders in hemodynamics, excretory function and urine concentrating ability of kidney during the early phase of reperfusion following renal ischemia.

 
Keyword(s): RENAL ISCHEMIA, ADENOSINE A1-RECEPTOR, INOS
 
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