Paper Information

Title: 

EFFECTS OF GRAPE SEED EXTRACT ON ISCHEMIA-REPERFUSION INDUCED INFARCT SIZE OF ISOLATED RAT HEARTS

Type: POSTER
Author(s): VAEZ H.*,ZAHEDNEZHAD F.,SAMADZADEH MEHRABAN,NAJAFI MOSLEM,BABAEI HOSSEIN
 
 *SCHOOL OF PHARMACY, TABRIZ UNIVERSITY OF MEDICAL SCIENCE, TABRIZ, IRAN
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2009Volume 19
 
 
Abstract: 

As no studies performed about pharmacologic effects of grape seed extract (GSE) on ischemia/reperfusion-induced injuries especially infarct size, we performed this study to investigate effects of GSE on isolated rat hearts. Male SD rats were divided into four groups (n=8 in each group) and isolated hearts mounted on Langendorff apparatus under constant pressure and temperature. After stabilization (30 min), regional ischemia induced for 30 min followed by 120 min reperfusion. In the control group, the hearts were perfused with extract free Krebs-Henseleit (K/H) solution, while in the test groups; they were perfused 1, 10 and 100 mg/l of GSE enriched K/H solution from 15 min before ischemia to 120 min reperfusion. Then the hearts colored by evans blue and incubated by triphenyl tetrazolim chloride (TTZ). The area of infacted tissue was determined by computerized planimetry. The results show that perfusion of GSE produced significant reduction in infarct size from 31±5% in control group to 12±3%, 16±6% (p<0.05) and 2±1% (p<0.01) in the test groups of GSE (1, 10 and 100 mg/l). The results can be attributed to the protective effects of proanthocyanidins to their ability to eliminatehydroxyl radicals. Also they may interact with intracellular calcium ions, leading to a reduction in reperfusion-induced calcium overload.

 
Keyword(s): GRAPE SEED EXTRACT, ISCHEMIA-REPERFUSION, INFARCT SIZE, ISOLATED RAT HEARTS
 
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