Paper Information

Title: 

EVALUATING THE EFFECT OF MEBUDIPINE ON STRESS OXIDATIVE AND ANTIOXIDANT DEFENSE IN MYOCARDIAL ISCHEMIC-REPERFUSION INJURY IN MALE RAT

Type: POSTER
Author(s): GHIASI R.*,MOHAMMADI MOSTAFA,BADALZADEH R.,VATANKHAH A.M.,ALVANDIAN E.,EBRAHIMI HADI,NAGHINEZHAD A.
 
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Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2009Volume 19
 
 
Abstract: 

Previous studies have indicated that several factors such as increasing of intracellular calcium, enhancement the production of free radicals and decreasing of antioxidant enzymes have important role in induction of myocardial ischemia-reperfusion injury. In this study, the effect of mebudipine as a new dihydropyridine calcium channel blocker, on oxidative stress in ischemia-reperfusion injury of isolated rat heart was investigated. Twenty-eight male Wistar rats (250-300g) were divided in four groups (n=7): Sham (without ischemia). control-ischemic mebudipine-ischemic and vehicle-ischemic groups. The animals were anesthetized by sodium pentobarbital (60 mg/kg–ip). The hearts were removed quickly and mounted on Longendorff apparatus and perfused by Krebs-Henseleit solution under constant pressure at 37oC. After 20 min stabilization period, ischemic groups were received 30 min global ischemia and 120 min reperfusion. In the mebudipine and vehicle groups, 25 min before ischemia the hearts were perfused with mebudipine (10-4μM) or ethanol (0.01%)-enriched solution for 25 min, respectively. At the end of experiment tissue samples were prepared from left ventricle. Malondialdehyde (MDA), creatine kinase (CK), lactate dehydogenase (LDH), superoxide dismutase (SOD), Glutathione peroxidase (GPX) and catalase levels were determined by using specific Kits. Mebudipine enhanced antioxidant enzymes (SOD, GPX and CAT) and decreased levels of LDH, CK and MDA as compared with those of control group (p<0.01). In conclusion, mebudipine by reducing the oxidative stress and enhancement of antioxidant defense reduced the intensity of myocardialischemiareperfusion injury and as a result, it may have beneficial effects in prevention of the ischemic side effects following cardiac infarctions.

 
Keyword(s): MEBUDIPINE, ISCHEMIA-REPERFUSION INJURY, ISOLATED HEART, OXIDATION STRESS
 
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