Paper Information

Title: 

THE EFFECT OF MEBUDIPINE ON ELECTRICAL ACTIVITY AND MYOCARDIAL ARRHYTHMIAS IN ISOLATED RAT HEART INJURED BY ISCHEMIA-REPERFUSION

Type: POSTER
Author(s): BADALZADEH R.*,MOHAMMADI MOSTAFA,GHIASI R.,SEPEHRI GH.R.,SHOARIAN Z.
 
 *DRUG APPLIED RESEARCH CENTER, SCHOOL OF MEDICINE, TABRIZ UNIVERSITY OF MEDICAL SCIENCES, TABRIZ, IRAN
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2009Volume 19
 
 
Abstract: 

One of the acute and lethal side-effects of reperfusion in ischemic area of the myocardium is electrical dysfunction and production of the severe arrhythmias. In this study, the effect of mebudipine as a new dihydropyridine calcium channel blocker, on electrical activity and myocardial arrhythmias in isolated rat heart injured by ischemia-reperfusion was investigated. Twenty-eight male Wistar rats (250-300g) were divided in four groups (n=7): sham (no-ischemia), controlischemic, mebudipine-ischemic and vehicle-ischemic groups. The animalsanesthetized by sodium pentobarbital (60mg/kg–ip). The isolated hearts mounted on Longendorff apparatus were perfused by Krebs-Henseleit solution under constant pressure at 37Co. After 20min stabilization period, ischemic groups were received 30 min global ischemia and 120min reperfusion. In the mebudipine and vehicle groups, before ischemia the hearts were perfused with mebudipine (10- 3μM) or ethanol (0.01%)-enriched solution for 25 min, respectively. Electrocardiogram (ECG) was recorded by using two golden electrode attached to the cardiac surfaces and one reference electrode. The ECG findings were interpreted according to Lambeth convention. The premature ventricular complexes (PVC), bigeminy and salvos counts as well as the numbers and duration of ventricular tachycardia (VT) and ventricular fibrillation (VF) in the reperfusion phase of control group were significantly increased as compared with the pre-ischemic period (p<0.01). Pre-treatment with mebudipine before ischemic phase remarkably reduced the number, duration and severity of myocardial arrhythmias and the incidence of VT and VF (p<0.05).There was no significant difference in HR among all groups. Our results indicate that mebudipine is effective in reducing the severity and numbers of arrhythmias-induced by myocardial ischemia-reperfusion injury and it may have anti-arrhythmic effects in the reperfusion phase.

 
Keyword(s): MEBUDIPINE, ISCHEMIA-REPERFUSION INJURY, ARRHYTHMIAS, ISOLATED HEART
 
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