Paper Information

Title: 

CARDIOPROTECTIVE EFFECTS OF CYNODON DACTYLON (L.) PERS. ON MYOCARDIAL ISCHEMIA AND REPERFUSION INJURY IN ISOLATED RAT HEARTS

Type: SPEECH
Author(s): GHAVIMI H.*,NAJAFI MOSLEM,GHARAHKHANI A.,GARJANI A.R.,NAZEMIEH H.
 
 *STUDENT RESEARCH COMMITTEE, TABRIZ UNIVERSITY OF MEDICAL SCIENCE, TABRIZ, IRAN
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2009Volume 19
 
 
Abstract: 

Although in Azerbaijan province folk medicine, Cynodon dactylon (L.) pers. (C. dactylon) rhizomes are used for treatment of cardiovascular disease, but its effect on heart hemodynamic factor and infarct size are not clear yet. Thus in the present study, effects of C. dactylon rhizomes total extract on ischemia/reperfusion (I/R) induced cardiac infarct size and hemodynamic factors were investigated in isolated rat heart. Isolated rat hearts from male Sprague Dawley rats (270-300 g) were mounted on a langendorff apparatus under 100 mmHg pressures at 37.5oC and perfused with modified Krebs-Henseleit (K/H) solution (in control group throughout the experiment) or enriched K/H solution with extract of C. dactylon rhizome (25, 50, 100 and 200μg/ml) during I/R, that previously equilibrated with 95% O2 -5% CO2. A fluid filled balloon was introduced into the left ventricle and inflated to give a preload of 8-10mmHg. Regional ischemia was induced by occlusion of left anterior decending coronary artery then by reoccluding of the coronary artery, the hearts allowed to be reperfused. Hemodynamic factors including left ventricular developed pressur (LVDP), heart rate (H.R) and rate pressure product (RPP) were measured at regular intervals by powerlab system (AD Instruments, Australia). Coronary flow rate (CFR) was measured by a time collection of the coronary perfusate. At the end of reperfusion, the heart were stained by Evans blue solution then incubated by Triphenyltetrazolium chloride. The volume of infarct tissue, risk zone and the percentage of infarct size were determined by computerized planimetry. In the control group, infarct size was34.3±2.3%, while perfusion of C. dactylon extracts (25, 50, 100 and 200μg/ml) reduced it to 4.9±2.2 (p<0.001), 13.4±3.4 (p<0.001), 20.5±3.6 (p<0.01) and 32±3.7% (p>0.05), respectively. Reduction of infarct size was reversely dependent on the concentration of extract. In the ischemia phase, perfusion of 25 and 50 μg/ml of extract enriched K/H solution had significant increase on LVDP and RPP in comparison with control group (p<0.05), however, higher concentration of extract had no significant effect on those factors. Any concentration of extract hadn't significant effects on CFR in the ischemia phase. Similar to ischemia, in the reperfusion phase perfusion of 25 and 50 μg/ml of extract enriched K/H solution had significant increase on LVDP and RPP in comparison with control group (p<0.05). In reperfusion time all concentration of extract had significant decrease in CFR (P<0.05). Result of this study showed that C .dactylon may protect against I/R injuries as reduction of infarct size. Improvement of some hemodynamic parameters in the isolated hearts may be involved in the protective effects of C. dactylon.

 
Keyword(s): CYNODON DACTYLON (L.) PERS. EXTRACT, MYOCARDIAL ISCHEMIA-REPERFUSION, INFARCT SIZE, HEMODYNAMIC FACTORS
 
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