Paper Information

Title: 

CYTOTOXIC EFFECT OF PROPRANOLOL ON HUMAN LEUKEMIC MOLT-4 CELL LINE

Type: SPEECH
Author(s): HAJI GHASEMI FATEMEH*,MIRSHAFIEI A.,POURPAK Z.
 
 *DEPARTMENT OF BIOLOGY, FACULTY OF BASIC SCIENCE, SHAHED UNIVERSITY, TEHRAN, IRAN
 
Name of Seminar: BIENNIAL CONGRESS ON IMMUNOLOGY, ASTHMA AND ALLERGY
Type of Seminar:  CONGRESS
Sponsor:  IRAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES
Date:  2007Volume 6
 
 
Abstract: 

Introduction: Propranolol, a beta-adrenergic blocker has been used for the treatment of a large number of cardiovascular diseases. This drug is also an inhibitor of phosphatidic acid (PA) phosphohydrolase and phosphatidic acid biosynthesis. Phosphatidic acid is a growth factor for tumor cells. In addition, the inhibitory effects of Propranolol on the development of a tobacco-induced pulmonary adenocarcinoma and also its cytotoxicity on rat and human lung macrophages and human lung tumor cell line have been reported. The widespread and long-term use of propranolol in lots of heart diseases as well as its cytotoxicity against some tumor cells prompted us to investigate its cytotoxic effect on a human T leukemic cell line (MOLT-4).
Materials and Methods: The MOLT-4 cells were cultured in complete RPMI medium and then incubated with different concentrations of Propranolol (0.0004 -0.4mM) for 10 and 20 hours. The cytotoxicity was then assessed by 3-[4,5-dimethyl thiazol-2,5-diphenyltetrazoliumbromide (MTT) reduction and also trypan blue dye exclusion methods.
Results: Propranolol induced a significant dose-dependent cytotoxic effect on human MOLT-4 cell line in less than 10 hours compared to untreated control cells.
Discussion: The results showed that human T leukemic cell line was dose-dependently-sensitive to Propranolol. Further studies investigating the in vivo effect of Propranolol on leukemic patients and also other leukemic cells are warranted.

 
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