Paper Information

Title: 

DESIGNING A HIGHLY EFFICIENT ARTIFICIAL CHAPERONE SYSTEM USING CHITOSAN-COATED ALGINATE

Type: POSTER
Author(s): FARAHMAND SH.,REZAEI NEGIN,KHODAGHOLI F.
 
 
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2009Volume 19
 
 
Abstract: 

Nowadays, the development of a protocol for the efficient refolding of the recombinant proteins has become an important issue because a variety of genetically engineered proteins for use in medical and bioindustrial areas aren usually produced in inactive aggregate forms called inclusion bodies. Our laboratory has investigated stability and refolding of denatured alkaline phosphatase (ALP), as a model by using alginate and chitosan-coated alginate. Alginate is regarded as a biocompatible, nontoxic, non-immunogenic and biodegradable polymer, making it an attractive candidate for biomedical applications. When an alginate drop falls into a chitosan solution containing a suitable divalent cation, the interphasic membrane is formed by complexation between the positively charged amine groups of chitosan and the negatively charged carboxyl groups of alginate through electrostatic interactions. Our results showed that in the presence of chitosan-coated alginate, ALP can be stabilized (in different PH and temperatures) and refolded to a higher degree compared with alginate microcapsules. Although alginate gel is also suitable, the environmental conditions could change the gel structure resulting to poor gel strength. Based on our results the protein refolding yield increased from 20% to 50% and 80% in the presence of alginate and chitosan-coated alginate respectively. In parallel, the remained activity increased from 3% to 30% and42% at 80°c in the presence of alginate and chitosan coated alginate respectively. In addition, choice of a suitable divalent ion was important for designing a suitable chaperone. In our work, the efficiency of them was in the following order: Cu>Ba>Ca>Co>Zn. The results of fluorescence also confirmed these data.

 
Keyword(s): 
 
Yearly Visit 20   tarjomyar
 
Latest on Blog
Enter SID Blog