Paper Information

Title: 

TERATOGENIC EFFECTS OF TESTOSTERONE ENANTATE ADMINISTRATION DURING PREGNANCY ON FETAL GENITALIA IN RAT

Type: POSTER
Author(s): MOGHADASI GH.R.,BEHNAM RASOULI MORTEZA,MAHDAVI SHAHRI N.
 
 
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2009Volume 19
 
 
Abstract: 

Clinically, androgenic drugs are prescribed in some gynecologic problems. However, administration of androgens in unaware pregnancies or abuse of synthetic androgens during pregnancy can lead to malformation and malfunction. The goal of the present study was to examine the effects of testosterone enantate administration during the critical period of reproductive tract development on the fetal genitalia in Wistar rat. Mated Wistar rats were divided into four groups: control and experimental groups 1, 2 and 3. At day 5 post-gestation, the pregnant rats of experimental groups 1, 2 and 3 received a single injection (i.m.) of 2, 4 or 6 mg testosterone enantate, respectively. On the 20th day of pregnancy and after the collection of blood samples for testosterone assay, all pregnant rats were sacrificed, their male and female fetus were sampled, fixed, processed for histological preparation, embedded in paraffin, serially sectioned and then the sampled sections stained with H & E. Compared to control group, the macroscopic study of female embryos belonging to the experimental groups showed musculinization, long anogenital distance, a scrotum like evagination and a large clitoris. Also there was some twisting in uterus tube of some female embryos. In theexperimental male e mbryos, the testicles and penises were bigger than controls. The microscopic study showed a mass of interstitial tissue and albuginea thickness around thetesticles males of experimental group 3.Furthermore, the blood vessels in the interstitial tissue and connective tissue under the albuginea grew more. However, there was not any difference between the ovaries of control and experimental groups. It seems that an increase in the level of maternal testosterone not only exerts teratogenic effects on the fetal development, but also affects the genital differentiation.

 
Keyword(s): TESTOSTERONE ENANTATE, GENITALIA, PREGNANCY, RAT
 
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