Paper Information

Title: 

AN EXPERIMENTAL ALLERGIC MODEL BY COMPLETE EXTRACT OF CUCUMIS MELO

Type: PAPER
Author(s): BODOUHI S.*,KAMALINEZHAD MOHAMMAD,AMINI D.,AMANI D.,MOSAFA NARIMAN
 
 *DEPARTMENT OF IMMUNOLOGY IN MEDICAL FACULTY, DEPARTMENT OF HERBAL MEDICINE IN PHARMATICS, FACULTY IN SHAHID BEHESTI UNIVERSITY OF MEDICAL SCIENCES
 
Name of Seminar: BIENNIAL CONGRESS ON IMMUNOLOGY, ASTHMA AND ALLERGY
Type of Seminar:  CONGRESS
Sponsor:  IRAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES
Date:  2007Volume 6
 
 
Abstract: 

Objective: One of the major sources of allergic components is fruit. Modern Aechniue and methods has been tried to separate those for diagnostic approach to allergy clinics and also for established experimental allergic models in laboratory and therapeutics goals. Extract of cucumis melo consist “cucumisin” which is a very potent allergen. We decided to use total extracts of cucumis melo for experimental animal model of allergy.
Material and Methods: 3 groups of animals (6 Inbred Balb/c mice) were used for this experimental study. The animals administered orally by complete extract of fruit mixed in drink water for 12 hours daily (according to appropriate).
Concentration: (1/2, 1/4, 1/8). After 2 weeks with appearance of erythema and pruitus in skin, the animals were scarified with euthanasi technique and then we prepared blood samples for peripheral evaluation of basophilli and serum IgE level measurement. Excise some pieces of duodenum and skin sample for histological determination of tissue eosinophils and basophils and pathological studies
Result: we detected great systemic basophile in all of the samples (up to 15%) in blood circulation. Another definds will be present in congress time.
Discussions and conclusion: Appearance of systemic and sever basophilli leads us to establish and induction of an experimental allergic model for pharmacological study in allergy. If we can prove the increased serum level of IgE and measurement of skin/intestinal mucosal basophil and audiophile, we deduce and will be successful in our study. Purification and isolation of crude extract to 14, 36, 54 and 67 KD of cucumisin leads us to a more specific Allergic and Experimental model in laboratory investigations.

 
Keyword(s): 
 
Yearly Visit 50   tarjomyar
 
Latest on Blog
Enter SID Blog