Paper Information

Title: 

SPASMODIC EFFECTS OF SENNA (CASSIA ITALICA) LEAVES AQUEOUS EXTRACT ON NON-PREGNANT UTERUS IN RATS

Type: POSTER
Author(s): OROUJAN ALI AKBAR*,AHANGARPOUR A.
 
 *DEPARTMENT OF PHYSIOLOGY, PHYSIOLOGY RESEARCH CENTER & DIABET RESEARCH CENTER, SCHOOL OF MEDICINE, JUNDISHAPOUR MEDICAL SCIENCES UNIVERSITY OF AHWAZ, AHWAZ, IRAN
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2009Volume 19
 
 
Abstract: 

Senna (Cassia italica) is a wild plant distributed throughout the Arabian Peninsula. Observed in the highest dose of Senna (dose producing a laxative effect) were an increased of water consumption, changes in electrolytes in serum and urine and increase of tubular basophiles and epithelial hypertrophy in the kidneys. In traditional medicine this plant has been used for the dysmenorrhea Therefore we decided to assay the effect of aqeous extract on uterus contraction.
Virgin rats from Wistar descent 200-300g were kept. After laparatomy, a piece of uterus (1.5cm) was excised and mounted in an organ bath (10ml) containing De Jalon solutions (29oC) less than 1g resting tension. KCl (60mM) induced uterus contraction and after 2min and during the plateau, the extract was added at 0.5, 1, 2 and 4 mg/ml cumulatively to the organ bath with 10 min intervals and no refreshing bath solution and the contractions was perused. The new portions of the uterus were separately incubated with atropine (10μM), metoclopramide (60μM) and oxytocin and the tissue spasmodic effect of the extract were recorded.
The results were analyzed by ANOVA and post hoc LSD tests. Cumulative concentrations of the extract (0.5-4 mg/ml) increased the basal and peak and frequency of uterus contractions, dose-dependently (P<0.001). Incubation of the tissue with atropine and metoclopramide did not reduce the spasmodic effect of the extract, but it was not dosedependently. Cassia italica extract was shown the oxytocic activity on the uterine smooth muscle which was less potent than that of oxytocin.
In conclusion, our study showed that the effect of aqueous extract of Cassia italica stimulated the uterus contractions without involving dopaminergic (D2), and mucarinic receptors. Probably activation of ATPdependent potassium channels partially was involved in this process.

 
Keyword(s): CASSIA ITALICA, ATROPINE, METOCLOPRAMAID, OXYTOCIN, UTERUS
 
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