Headache is a common pain clinical problem. Following neurogenic headache, migraine is the most prevalent type of headache. The prevalence of migraine is18% in women and 6% in men. This sexual difference appears in menarch. Recent studies have shown that sexual hormone variations in patients can play role in migraine. In this study 30 women among patients that referred to neurology clinic and 30 healthy women as control group with the same age range were studied. Measuring of blood pressure, height, weight and blood sampling were performed on the 5^th and 25^th days of the cycle. Progesterone level significantly increased on the 25^th day in patient group (p<0.0086). Increased level of progesterone from the 5^th to 25^th days in patient group significantly increased compared to control group (P<0.0072). Ratio of estrogen to progesterone significantly decreased on the 5^th and 25^th days in patient group (P<0.078). According to this study, increased level of progesterone on the 25^th day in patient group was more than control group and increased level of estrogen did not show a significant difference between two groups, although previous studies have shown a decrease in estrogen before menstrual was essential factor. In addition, contrary to recent studies, the ratio of estrogen to progesterone on the 25th day in patient group showed a fall rather than rise. Therefore, this study suggests that progesterone has a role in migraine.