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Paper Information

Title: 

THE ANTIBACTERIAL & ANTIFUNGAL EFFECT OF AQUEOUS AND ORGANIC EXTRACTS (CONSIST OF: CHLOROFORM, METHANOL, ETHYL ACETATE, PETROLEUM ETHER) OF PRUNUS ARMENIACA L. LEAF & GUM ON SEVEN SPECIES OF BACTERIA & FUNGI

Type: POSTER
Author(s): KARANJAM S.Z.*,NAJI T.,KAMALINEZHAD MOHAMMAD
 
 *PHARMACEUTICAL SCIENCE BRANCH, ISLAMIC AZAD UNIVERSITY, TEHRAN, IRAN
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2009Volume 19
 
 
Abstract: 

Background & Objectives: During the last two decades, due to rapid development of drug resistance in pathogenic microorganisms as well as the appearance of undesirable side effects of some antibiotics , various medicinal herbs have beeen studied for presence of antibacterial activity to discover new antibacterial agent capable of resolving these problems.According to Iran’s geographic situation , we see a great terms of growthing of many plants.One of the most important of plant’s family is Rosacea. In this study the antimicrobial activity of aqueous and organic (A and O) extracts of leaf and gum of Prunus armeniaca L. was investigated.
Material and Method: The air-dried leaf and gum material were extracted by maceration method and using the solvent (Chloroform, methanol, ethyl acetate, petrolim ether and water). The A and O extracts were tested at concenterations of 400mg/ml, 300mg/ml and 200mg/ml against some gram-positive ( g +) and some gram-negative (g -) bacteria and fungi by Kirby-Bauer method and using disk diffusion technique. At the end assessing the MIC using microplate method.
Staphylococcus aureus (PTCC 1112)
Staphylococcus epidermidis (PTCC 1114)
Streptococcus pyogenese (PTCC 1447)
Klebsiella pneumonia (PTCC 1053)
Escherchia coli (PTCC 1399)
Pseudomonas aueroginosa (PTCC 1074)
Candida albicans (PTCC 1053)

 

Extracts

 

Concentrations

Inhibition

Zone Of

C.albicans

Inhibition

Zone Of

P.aueroginosa

Inhibition

Zone Of

E.coli

Inhibition

Zone Of

K.pneumonia

Inhibition

Zone Of

S.pyogenese

Inhibition

Zone Of

S.epidermidis

Inhibition

Zone Of

S.aureus

 

Metanolic Gum

400mg/ml

20mm

25mm

15mm

15mm

15mm

16mm

21mm

300mg/ml

17mm

24mm

12mm

10mm

13mm

11mm

17mm

200mg/ml

15mm

19mm

11mm

7mm

10mm

10mm

15mm

 

Watery

Gum

400mg/ml

10mm

16mm

9mm

7mm

14mm

12mm

8mm

300mg/ml

9mm

13mm

7mm

6mm

13mm

9mm

7mm

200mg/ml

7mm

11mm        

6mm

6mm

11mm

8mm

7mm

Results and discussion: As the results of antimicrobial effects of our plant we recommended more investigation tests on other part of this plant such as fruit.
The other results will be presented at Seminar

 
Keyword(s): PRUNUS ARMENIACA L., AQUEOUS AND ORGANIC, KIRBY-BAUER METHOD, MIC
 
 
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