Paper Information

Title:  IN VITRO ANTI-BACTERIAL ACTIVITY OF METHANOL EXTRACT OF POMEGRANATE (PUNICA GRANATUM L.) PEEL ON HELICOBACTER PYLORI ISOLATES
Type: POSTER
Author(s): NAKHAEI MOGHADAM M.*,ROUHANI SH.
 
 *DEPARTMENT OF BIOLOGY, ISLAMIC AZAD UNIVERSITY, MASHHAD BRANCH
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2009Volume 19
 
 
Abstract: 

Pomegranate peel has been used for many years to treat diarrhea, peptic and duodenal ulcer. Helicobacter pylori (HP) infection is the most common gastrointestinal bacterial disease worldwide. It is known that HP could be eradicated by a combination of therapeutic agents, but sometimes the cure thus achieved is incomplete. Also, undesirable side effects are certain to occur. In this study we decided to evaluate the activity of pomegranate peel against H. pylori isolates. Macerated methanol extract of peel was tested against 27 clinical isolates of HP. Growth inhibition was determined by filter paper disc diffusion method on modified egg yolk emulsion agar (EYE agar) compared with amoxicillin (25μg/disc) and metronidazole (5μg). MIC was determined by agar dilution. The growth of all isolates was inhibited by 2mg of methanol extract. The mean±S.E of inhibition zone of extract was 27.96±0.97 mm. The minimum inhibitory concentration (MIC) was 312.5μg/ml and minimum bactericidal concentration (MBC) of extract was 338.5μg/ml. Methanol extract preserved its anti- Helicobacter pylori activity after autoclaving (121ºC for 20min) and it was stable at pH 5-7. Chloroform fraction of extract had no anti-Helicobacter pylori activity. The mean of inhibition zone of butanol fraction (25.3±0.86) was more than aqueous fraction (22.69±0.82) but there is no difference (p>0.05) between their anti-Helicobacter pylori activity of them based on Duncan test.
This study demonstrated that pomegranate peel inhibited the growth of tested HP isolates in vitro. Research about fractionation and purification of pomegranate constituents and in vivo anti-bacterial activity of this extract is recommended.

 
Keyword(s): PUNICA GRANATUM, HELICOBACTER PYLORI, ANTI-BACTERIAL, POMEGRANATE PEEL
 
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