Paper Information

Title: 

EVALUATION OF ANTIMICROBIAL EFFECTS OF VARIOUS ORGANIC AND AQUEOUS EXTRACTS OF TRIBULUS TERRESTRIS L. FRUITS

Type: POSTER
Author(s): MEHREGAN HADI,HEYDARZADEH J.*,AYAT ELAHI S.A.A.M.,KAMALINEZHAD MOHAMMAD,MOHAMMADZADEH MAHDI
 
 *DEPARTMENT OF PHARMACOGNOSY, SCHOOL OF PHARMACY, SHAHID BEHESHTI UNIVERSITY (M.C), TEHRAN, IRAN
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2009Volume 19
 
 
Abstract: 

Infusion of T. terrestris or Caltrop fruits has been long used for the treatment of ailments such as urinary tract infections. Antimicrobial activity of various extracts of Caltrop fruits was thus investigated in this study. Caltrop fruits were obtained from Shahryar city, South West of Tehran, in September 2008 and shade dried. Fruit powder was then extracted by the maceration method using water, methanol, ethyl acetate, chloroform and hexane as solvents. The dried extracts were reconstituted using DMSO at both 10 and 100 mg/ml and antimicrobial activities were evaluated against an array of 15 Gram-positive (GPB) and Gram-negative (GNB) bacteria as well as 2fungi (Candida albicans and Aspergillus niger) by the cup-plate method.Minimum inhibitory concentrations (MIC) of the extracts were also determined by the Agar dilution technique (CLSI 2006).
The extracts nearly always showed no activity against GNB. On the other hand, these extracts showed a weak activity against some GPBs such as Enterococcus feacalis and Listeria monocytogenes (zone diameter: 10-16 mm) and a significant activity against some other GPBs like Staphylococci and Micrococcus luteus (zone diameter: 18-24 mm). Chloroformic extract at 10 mg/ml had the highest activity on GPBs. MIC values obtained were 800 μg/ml. Furthermore, these extracts showed no activity against test fungi. Negligible effect of the extracts against GNBs could be attributed to their Outer membrane, which impedes the penetration of materials. None of the extracts could inhibit bacterial growth at 100 μg/ml. Based on our findings, Caltrop fruits appeared not to have significant antimicrobial effects; These results are in line with some reports but differ with others.

 
Keyword(s): TRIBULUS TERRESTRIS, CALTROP, ANTIMICROBIAL EFFECT, MACERATION TECHNIQUE
 
Yearly Visit 23   tarjomyar
 
Latest on Blog
Enter SID Blog