Paper Information

Title: 

MORPHINE EXPOSURE DECREASES BRAIN TESTOSTERONE LEVELS THROUGH INCREASE IN 5α-REDUCTASE TYPE 1 GENE EXPRESSION IN THE ADULT MALE RATS

Type: POSTER
Author(s): VERDI J.*,AHMADIANI A.A.H.
 
 *DEPARTMENT OF PHYSIOLOGY AND PHARMACOLOGY, SCHOOL OF MEDICINE, KASHAN UNIVERSITY OF MEDICAL SCIENCES, KASHAN, IRAN
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2009Volume 19
 
 
Abstract: 

The enzyme 5alpha–reductase (5a-R) converts testosterone to dihydrotestosterone. In the central nervous system there exist two isoforms of this enzyme wiz, 5a-R1 and 5a-R2. It is the isoform 5a-R1 which essentially plays a catabolic and neuroprotective role in the brain. We previously indicated that morphine administration increases 5a-R activity in the brain. In this work, we studied the effect of morphine on brain testosterone levels and also evaluated the effects of acute (10 mg/kg, i.p.) and chronic (20 mg/kg, once daily for 4 days, i.p.) morphine on the 5a-R1-gene expression in the adult male rate brain, using an RT-PCR method. The results of our study revealed that morphine could significantly reduce the testosterone concentration in the brain and that the level of 5a-R1-gene expression increased following both acute and chronic morphine administration. More over, tolerance to increasing effect of morphine on 5a-R1 gene expressions is developed in chronic administration. We concluded that the decreasing effect of morphine exposure on the brain testosterone levels is through an increase in 5a-R1 gene expression a possibly due to the effect of morphine as transcription level and/or translation level. Therefore, morphine may play a regulatory role in metabolism of neurosteroids, especially testosterone, in the brain.

 
Keyword(s): MORPHINE, TESTOSTERONE, BRAIN, 5 ALPHA-REDUCTASE, GENE EXPRESSION, RAT
 
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