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Paper Information

Title: 

EVALUATION OF PROBABLE PROTECTIVE EFFECTS OF SPECIFIC CGMP PHOSPHODIESTERASE INHIBITORS ON LEAD-INDUCE LIVER TOXICITY AND LIPID PER OXIDATION IN A RAT LIVER PERFUSION MODEL

Type: SPEECH
Author(s): AGHABABAEI R.,ABDOLLAHI M.,GHAZI KHANSARI M.,ABDI KH.
 
 
 
Name of Seminar: IRANIAN CONGRESS OF TOXICOLOGY
Type of Seminar:  CONGRESS
Sponsor:  SOCIETY OF TOXICOLOGY
Date:  2004Volume 8
 
 
Abstract: 

Lead is one of the most important pollutants of mankind's environment enters into the body via Respiratory and gastrointestinal tract and distributed into liver, kindly and soft tissues after absorption and is accumulated finally in bones. Lead effect different system of body, namely immune and liver system. This is one of the characteristic of lead that results in its accumulation and chronic toxicity. Though, the lead is one of the most important heavy metals under the investigation in recent years, no clear mechanism for its toxicity has been mentioned and proven. The proposed perfusion system is very close to normal and natural physiologic condition of the body is used most frequently in hepatic in vitro studies. In this method the hepatic cells were exposed directly to different concentration of chemical agents. Rat liver is per fused with Krebs-Hansel it solution method and lead chloride solution in 0.1 mg/L concentration during 1 hr. The concentration transaminase (AST, ALT), lipid peroxide enzymes content of perfused solution and total protein in homogenized hepatic tissues are used as an indication of hepatic toxicity. To reduce the lead toxic effect, the preservation activity of dipyridamol in 50 µM dose is used in a combination therapy and pre-treatment manner separately. The studied compounds are administered 25 min in pre-treatment mode and concurrently in combination therapy mode too. The result showed that the transaminase enzymes in perfused hepatic sample did not show a significant difference in lead and control group. The results showed also that the dipyridamol decreased lipid per oxidation of the pre-treatment and combination therapy group significantly in comparison to lead group (p<0.001). The studies showed that the lead causes oxidative stress in liver, and also increases the lipid per oxidation, reduction of thiol group's level and anti oxidative strength. Lead dependent oxidative stress mechanism is not quite clear but it is clear that the oxidative stress inducer agent decreases cellular damage anti-oxidative defense system that leads to its ultimate cellular damage and systemic disorders.

 
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