Click for new scientific resources and news about Corona[COVID-19]

Paper Information

Title: 

ISOBOLOGRAM GUIDELINE FOR TAXOL AND CISPLATIN SYNERGISTIC EFFECTS ON HUMAN LUNG CANCER A549 CELLS

Type: POSTER
Author(s): MOVASAGHIAN S.,HOSSEINI SHIRAZI S.F.
 
 
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2009Volume 19
 
 
Abstract: 

Despite recent improvements for cancer therapy, there has been no significant increase in long-term survival rates in lung adenocarcinoma patients. Cisplatin is a very common and effective chemotherapeutic agent for various carcinomas. However, its poor response in lung cancer, as well as resistance to cisplatin is serious problems to use this agent for lung cancerous patients. Paclitaxol is a good candidate for these patients, but in a very high cost and limited therapeutic response. Co-administration of different anticancer agents might be a good idea to overcome the problem. Only a few studies have looked into the synergistic outcome of cisplatin and taxol for the chemo-resistant human non-small cell lung carcinomas. We have therefore designed an isobologram study to investigate on the synergistic effects of these two agents on human lung cells. Human lung adenocarcinoma epithelial cell line (A549) was grown in DMEM/F12 culture media supplemented with 10% FBS in humified 5% CO2 incubator. Isobologram study was performing using 24 hours treatment of Cisplatin, Taxol and/or their combinations on this cell line. Cytotoxic effects were determined by MTT assay.
The theoretical additively curve was determined based on the cytotoxicity concentrations for 40% killing effects (LD40) of Taxol and Cisplatin on A549 cell line; exactly on 74 and 2100 μM, respectively. In the combined drug treatment, varying doses of Taxol (50, 74 and 100 μM) were added to the 2100 μM of Cisplatin. Similarly, a fix dose of 74 μM Taxol was used with the varying doses of Cisplatin (1500, 2100 and 3000 μM). Resultant connected line between the LD40s of these two curves have resulted in the isobologram guideline for the combination cytotoxic effects of these two drugs on A549 cell line, which will be presented in the meeting.

 
Keyword(s): CISPLATIN, TAXOL, ISOBOLOGRAM, LUNG CANCER
 
 
Yearly Visit 43   tarjomyar
 
Latest on Blog
Enter SID Blog